BOSTON - The U.S. Environmental Protection Agency (EPA) is proposing targeted modifications to the 2017 Small Municipal Separate Storm Sewer System (MS4) general permit for New Hampshire communities.

NOGALES, AZ – Hoy, la Agencia de Protección Ambiental de EE. UU.

SAN DIEGO – Hoy, la Agencia de Protección Ambiental de Estados Unidos (EPA, por sus siglas en inglés), en coordinación con el Banco de Desarrollo de América del Norte (BDAN), emitió una Solicitud de Propuestas (RFP, por sus siglas en inglés) a través del Programa Frontera 2020.

Environmental News FOR IMMEDIATE RELEASE

Sheboygan County, Wis. – Today, the U.S.

WASHINGTON (April 28, 2020) —  Today, the U.S. Environmental Protection Agency (EPA) and State of Indiana announced that recent air monitoring data show the city of Indianapolis, and the areas of Muncie, Lake and Porter counties are meeting federal air quality standards.

The C train reached its last stop just after 9 a.m. Saturday at the Euclid Ave. station in East New York when the man insisted he needed five more minutes before he could leave the car.

The C train reached its last stop just after 9 a.m. Saturday at the Euclid Ave. station in East New York when the man insisted he needed five more minutes before he could leave the car.

Metropolitan Police Federation chairman says planned 14-day strategy is unenforceable

Now you can read all of IndyStar's sports content in one place with our free INSports app.

Robbie Amos, 66, "ate, lived and breathed law enforcement," says his wife. He died on April 3 after contracting the novel coronavirus.

Decision makers at Penske Entertainment are beginning to chart the course for a historic summer of racing at the Indianapolis Motor Speedway.

Volunteers from six Santa Clarita Valley high schools step forward to help those shut in by the COVID-19 pandemic.

In the latest MOTD podcast the experts discussed the top 10 Ballon d'Or winners of the Premier League era - and here's how you ranked them.

While the weather may have looked like mid-November in Brockville Saturday morning, that didn't stop people from donating to the Brockville community food drive.

A polar vortex has blasted into the northeastern US this weekend bringing rare May snowfall and record low temperatures to some areas.

Members of the Joint Operational Committee in Tshwane is in self-isolation after one member tested positive for Covid-19, spokesperson Brigadier Vishnu Naidoo has told News24.

Flying in Canada during the time of COVID-19 requires a lot extra care, and CTV Senior Political Correspondent Glen McGregor gives a first-hand account on CTVNews.ca.

John Hillman - the 101-year-old Second World War veteran who has been walking laps around the courtyard of his Oak Bay retirement home in hopes of raising $101,000 for charity - has surpassed his goal.

Members Event

Event participants

G John Ikenberry, Albert G Milbank Professor of Politics and International Affairs, Princeton University; Eastman Professor, Balliol College, Oxford
Chair: Dr Robin Niblett, Director, Chatham House

Research Event

Event participants

Dr Maria Ângela Bragança, Secretary of State for Cooperation, Ministry of External Relations, Angola

Research Event

Event participants

Dr Doyin Okupe, Senior Special Assistant on Public Affairs to the President of Nigeria 

Invitation Only Research Event

21 July 2016

The government said it's distributing the promising coronavirus drug, remdesivir, to some hard-hit states. Eventually, all 50 states should get it.

Antibodies are widely used as cancer therapeutics, but their current use is limited by the low number of antigens restricted to cancer cells. A receptor tyrosine kinase, receptor tyrosine kinase-like orphan receptor 2 (ROR2), is normally expressed only during embryogenesis and is tightly down-regulated in postnatal healthy tissues. However, it is up-regulated in a diverse set of hematologic and solid malignancies, thus ROR2 represents a candidate antigen for antibody-based cancer therapy. Here we describe the affinity maturation and humanization of a rabbit mAb that binds human and mouse ROR2 but not human ROR1 or other human cell-surface antigens. Co-crystallization of the parental rabbit mAb in complex with the human ROR2 kringle domain (hROR2-Kr) guided affinity maturation by heavy-chain complementarity-determining region 3 (HCDR3)-focused mutagenesis and selection. The affinity-matured rabbit mAb was then humanized by complementarity-determining region (CDR) grafting and framework fine tuning and again co-crystallized with hROR2-Kr. We show that the affinity-matured and humanized mAb retains strong affinity and specificity to ROR2 and, following conversion to a T cell–engaging bispecific antibody, has potent cytotoxicity toward ROR2-expressing cells. We anticipate that this humanized affinity-matured mAb will find application for antibody-based cancer therapy of ROR2-expressing neoplasms.

Bacterial biofilms are cellular communities that produce an adherent matrix. Exopolysaccharides are key structural components of this matrix and are required for the assembly and architecture of biofilms produced by a wide variety of microorganisms. The human bacterial pathogens Escherichia coli and Salmonella enterica produce a biofilm matrix composed primarily of the exopolysaccharide phosphoethanolamine (pEtN) cellulose. Once thought to be composed of only underivatized cellulose, the pEtN modification present in these matrices has been implicated in the overall architecture and integrity of the biofilm. However, an understanding of the mechanism underlying pEtN derivatization of the cellulose exopolysaccharide remains elusive. The bacterial cellulose synthase subunit G (BcsG) is a predicted inner membrane–localized metalloenzyme that has been proposed to catalyze the transfer of the pEtN group from membrane phospholipids to cellulose. Here we present evidence that the C-terminal domain of BcsG from E. coli (EcBcsGΔN) functions as a phosphoethanolamine transferase in vitro with substrate preference for cellulosic materials. Structural characterization of EcBcsGΔN revealed that it belongs to the alkaline phosphatase superfamily, contains a Zn2+ ion at its active center, and is structurally similar to characterized enzymes that confer colistin resistance in Gram-negative bacteria. Informed by our structural studies, we present a functional complementation experiment in E. coli AR3110, indicating that the activity of the BcsG C-terminal domain is essential for integrity of the pellicular biofilm. Furthermore, our results established a similar but distinct active-site architecture and catalytic mechanism shared between BcsG and the colistin resistance enzymes.

Fucosylation of the innermost GlcNAc of N-glycans by fucosyltransferase 8 (FUT8) is an important step in the maturation of complex and hybrid N-glycans. This simple modification can dramatically affect the activities and half-lives of glycoproteins, effects that are relevant to understanding the invasiveness of some cancers, development of mAb therapeutics, and the etiology of a congenital glycosylation disorder. The acceptor substrate preferences of FUT8 are well-characterized and provide a framework for understanding N-glycan maturation in the Golgi; however, the structural basis of these substrate preferences and the mechanism through which catalysis is achieved remain unknown. Here we describe several structures of mouse and human FUT8 in the apo state and in complex with GDP, a mimic of the donor substrate, and with a glycopeptide acceptor substrate at 1.80–2.50 Å resolution. These structures provide insights into a unique conformational change associated with donor substrate binding, common strategies employed by fucosyltransferases to coordinate GDP, features that define acceptor substrate preferences, and a likely mechanism for enzyme catalysis. Together with molecular dynamics simulations, the structures also revealed how FUT8 dimerization plays an important role in defining the acceptor substrate-binding site. Collectively, this information significantly builds on our understanding of the core fucosylation process.

The EU-U.S. relationship is one of the most significant partnerships among wealthy nations. Interconnections between the two on migration issues make dialogue necessary and inevitable, as each relies on each other to attain a number of policy objectives, most clearly in the case of travel and border security.

When siblings Norma and Warren Williams left Jamaica to attend the funeral of a relative in Margate, South Florida, in February, they had no idea that a rapidly developing COVID-19 outbreak would have crippled global travel, leaving them stranded...

At least 10 stores inside the Portmore Mall have fallen casualty to the economic chokehold brought on by the COVID-19 pandemic, with several others struggling to stay afloat as St Catherine businesses grapple with revenue losses. And with a 14-day...

This is the latest in NCIIP’s language access webinar series exploring the policy and program implementation imperatives for government and community agencies serving Limited English Proficient (LEP) populations.

This webinar from the MPI’s National Center on Immigrant Integration Policy and the U.S. Conference of Catholic Bishops’ Bridging Refugee Youth & Children’s Services program explores federal requirements for providing interpretation and translation in schools and how select school districts in Minnesota and Colorado have managed these requirements.

This MPI webinar features U.S. Department of Justice (DOJ) officials discussing the department’s efforts to improve communications with Limited English Proficient (LEP) communities in federal and federally-funded programs and activities.

This MPI webinar features U.S. Department of Justice (DOJ) officials discussing the department’s efforts to improve communications with Limited English Proficient (LEP) communities in federal and federally-funded programs and activities.

The discovery of an inhibitory memory circuit could lead to novel treatments for conditions such as PTSD

In order to remember, we must forget. Recent research shows that when your brain retrieves newly encoded information, it suppresses older related information so that it does not interfere with the process of recall. Now a team of European researchers has identified a neural pathway that induces forgetting by actively erasing memories. The findings could eventually lead to novel treatments for conditions such as post-traumatic stress disorder (PTSD).

We’ve known since the early 1950s that a brain structure called the hippocampus is critical for memory formation and retrieval, and subsequent work using modern techniques has revealed a great deal of information about the underlying cellular mechanisms. The hippocampus contains neural circuits that loop through three of its sub-regions – the dentate gyrus and the CA3 and CA1 areas – and it’s widely believed that memories form by the strengthening and weakening of synaptic connections within these circuits.

Related: Light switches memories on and off | Mo Costandi

Related: The Homer Simpson effect: forgetting to remember

Why did right-winger Yosemite Sam have problem with the leftist Bugs Bunny?

Tesla filed a lawsuit Saturday against Alameda County, Calif., and CEO Elon Musk threatened to move the company's headquarters and operations out of state.

Ahead of National Citizenship Day, this commentary examines how regulations for the implementation of the federal Workforce Innovation and Opportunity Act (WIOA) at state and local levels could jeopardize citizenship preparation services for millions of immigrants across the United States.

Whether driven by pragmatism, local laws, or federal civil-rights provisions, state and local governments and agencies across the United States increasingly have designed and implemented language access services (i.e. translation and interpretation) in response to growing Limited English Proficient populations. This commentary argues it is time for the federal government to follow suit.

Drummer Owen Clark, 81, said in the 1950s, Manitoba teenagers would dance to Little Richard all night long. The 87-year-old rock pioneer died on Saturday morning.

Producer Karim Morani's daughter Zoa Morani donated her blood for plasmatherapy trials for COVID-19 treatment at Mumbai's Nair hospital today.

An Arctic cold air mass brought record low temperatures in Windsor and other cities around the province last night

For the past few weeks, Windsor's new pandemic response therapist Dana St. Jean has been spending her time fielding phone calls and speaking with people over video chat to find ways to help them cope with the stresses of COVID-19.

Ministries in Turkey experience acceleration through wide sowing, building projects, and church planting.