Death Stranding Director’s Cut - PlayStation 5 $10.00

https://www.bestbuy.com/site/death-stranding-directors-cut-playstation-5/6471392.p?skuId=6471392

Title says it all

https://www.antonline.com/Sony/Electronics/Gaming_Devices/Gaming_Consoles/1487917

Crisis Core: Final Fantasy VII Reunion

https://www.play-asia.com/crisis-core-final-fantasy-vii-reunion-multi-language/13/70fivr?tagid=16202

Abyss of the Sacrifice 

https://www.play-asia.com/abyss-of-the-sacrifice-multi-language/13/70dr1n?tagid=16202

Use coupon code FALLEN5
Free shipping
 
https://www.play-asia.com/lords-of-the-fallen/13/70g6lb

PlayStation $100 GC $90 w/coupon: FTTCX9645

Xbox $100 GC $88 w/coupon: FTTCX9426

Nintendo $50 GC $45 w/coupong: FTTCX9425

Sonic MotherBoard $189.99

All Gaming items at Fantastech Sale

Everything at NewEgg's Fantastech Sale

https://www.gamefly.com/preplayedsale

Use code gamefly05 for an additional 5% off for first purchase (or create new account with new email).

Some highlights:
Diablo IV $30
Final Fantasy XVI $35
Dead Island 2 $20
Star Wars Jedi Survivor $28
Hogwarts Legacy $30
RE4 $35
Dead Space $25
God of War Ragnarok $28
Forspoken $18
Midnight Suns $20
Last of Us Part I $35
Atomic Heart $28
Wild Hearts $25/$18
Horizon Forbidden West $28

https://www.play-asia.com/ea-sports-fc-24/13/70glbt

https://www.play-asia.com/resident-evil-4/13/70fhah

New on Men.com: On his way to the bedroom, Shadow is distracted by his girl’s (Cherry Kiss) roommate Chase‘s ass, but he is really aroused and has been wanting to pound his girlfriend all day. Shadow gets the gaymer to take over as Cherry Kiss has to halt the BJ to answer a phone call!... View Article

The post Men.com: Shadow, Chase and Cherry Kiss – “Played By The Straight Jock” appeared first on QueerClick.

In this episode I'm joined by Clive Palmer to chat about the kind of incoming transfer business Arsenal will look to get done, and why someone like Mikel Merino might not be the most exciting arrival, but someone who can easily plug and play into Mikel Arteta's system. We also chat about some potential departures, what we need to see from Fabio Vieira this season, Ethan Nwaneri and how pre-season could be very important for him, and lots more, including some US tour chat

Follow Clive @clivepafc

Get extra bonus content and help support Arseblog by becoming an Arseblog Member on Patreon: https://www.patreon.com/arseblog

Hosted on Acast. See acast.com/privacy for more information.

In this episode I'm joined by Lewis Ambrose to chat about last night's 4-1 friendly win over Bayer Leverkusen. We analyse the very familiar looking team line-up in the context of Mikel Arteta's comments about needing more from the players we have, leading to discussion of Gabriel Jesus and Kai Havertz among others. There's also some focus on young players like Myles Lewis-Skelly and Ethan Nwaneri after they impress again, but how do you find the balance between their integration and further additions to the squad? There's some transfer discussion, Arteta's pickpocketing high jinx, and a goodbye from an old (angry) friend.

Get extra bonus content and help support Arseblog by becoming an Arseblog Member on Patreon: https://www.patreon.com/arseblog

Hosted on Acast. See acast.com/privacy for more information.

About the Event Our resident ensemble of Princeton University performance faculty and talented students presents a Sunday-afternoon program of songs with and without words written by female composers on both sides of the Atlantic. Songs for mezzo-soprano and piano by prolific lieder composer Josephine Lang and for mezzo-soprano and mixed chamber ensemble by Dame Ethel Smyth bookend the program, with works for string quartet, solo piano, and flute, viola, and harp, that reference American, Jamaican, and European song and poetry in between. This event is presented by Princeton University Concerts. For a full event listing and tickets, please visit this link.

Not long ago, at bedtime, this discussion ensured: 2-year-old (excitedly): Jesus coming? Me: He’s probably not coming for a while,

Read More

The post Playtime with Jesus appeared first on Women of the ELCA.

The world’s best pump track riders descended upon a vibrant township on Durban’s north coast to battle for top honours.
( Photos: 9, Comments: 13 )

A Malaysian security department needed a system that their programmers could incorporate Q management control with digital signage software. They display Repeat Signage on their screens to show live TV, the counter and announcements, in this case study.

7-minute video shows you how to filter and display data in a datagrid as part of your digital signage software presentations for display on any screen, anywhere. This feature is available in Repeat Signage Corporate or Media Wall editions and is ideal for corporate offices to help keep staff updated on relevant information

7-minute video shows how you can a single line of text from either a .txt file or a database, for example, displaying name of duty manager. The ability to display database information is available in the Corporate and Media Wall editions of Repeat Signage digital signage software. Ideal for displaying company information on any screen anywhere.

From rejection at Benfica to new Manchester United head coach, BBC Sport charts the rise of Ruben Amorim.

Watch the best plays from week ten in the NFL, featuring an amazing catch by Pittsburgh Steelers wide receiver George Pickens.

The Prince of Wales is on a trip in South Africa promoting his Earthshot environmental prize.

1. Coldplay announces fourth show in Ahmedabad on 25 January. Tickets available from… | Today News  Mint
2. Coldplay announces new show in Ahmedabad: Check date, how to book tickets and more  India Today
3. Coldplay Announces Fourth Concert In India. Check Out Date, Venue And Ticket Prices  NDTV Movies
4. Coldplay announces new show in Ahmedabad, check details here  CNBCTV18
5. entertainment News Live Today November 13, 2024: Coldplay announced 4th India show in Ahmedabad: Check out ticket sale details  Hindustan Times

Philadelphia and other cities are quietly building installations like the “Urban Thinkscape” to layer on learning where families already spend time. On a tiny triangular lot in the city’s Belmont neighborhood, kids waiting with their parents for the No. 40 bus can also work on their executive functioning skills, playing a hopscotch variation designed to train their brains. In Chicago, a wooden game mounted on the wall of a laundromat teaches children, in two languages, how to find color patterns in a lineup of detergent bottle tops.

LG’s latest prototype screen is stretchy. Shown off at LG’s Science Park in Seoul, the company demonstrated the new type… Continue reading LG gives glimpse into the future with stretchy display

The post LG gives glimpse into the future with stretchy display appeared first on ReadWrite.

The PlayStation 5 Pro hits shops on Thursday with a price tag that has raised eyebrows among gamers, but growing sticker shocks in the tech industry have yet to deter consumers.

TL;DR: Get 37% off this touchscreen infotainment system with CarPlay and Android Auto compatibility. 

Elon, who?? Okay, maybe Teslas have cool infotainment systems, but you don't have to pay crazy prices for an over-hyped screen—just grab one of these CarPlay displays for your old beater. — Read the rest

The post Teslas suck! What you really need is this whizz-bang CarPlay screen appeared first on Boing Boing.

You can download the official release of Adobe Flash Player 10.1 on the Adobe site.

This is the first step in what will be an extremely eventful year for streaming media. Adobe will soon enter the enterprise market in a big way with a version of Flash Media Server that supports both IP and Application Multicasting.

Flash has long dominated the public internet, but its inability to multicast meant corporations had to stick with Windows Media. This new capability will give large enterprises the opportunity to migrate to the Flash format and benefit from its ability to run on any OS and any browser, ease of use, support for higher quality codecs, ability to change bit rates on the fly etc.

If anyone could accuse Trump of being on 'defense' during his witch-hunt trials, he's going on full offense now.

The post BOOM: Trump’s Lawyers SAVAGE Letitia James… Threaten PRISON If She Plays More Of Her Games appeared first on Clash Daily.

Sir Kenny Dalglish hailed nine-time Major champion Gary Player and expressed his delight at Liverpool's position at the top of the Premier League during his visit to South Africa this weekend.

Bafana Bafana coach Hugo Broos has welcomed the number of young players who are given a chance and backed in the premier division, but has warned that to build on their progress, agents must prioritise players' development over their personal enrichment.

The Springboks will continue to evolve their attack, but they must do so within the framework of what has made them so successful, says attack coach Tony Brown.

The MENA uprisings: Five years on, what role is civil society playing? 31 October 2024 — 2:00PM TO 3:00PM Anonymous (not verified) 23 October 2024 Online

Panellists examine the protest movements’ legacies in different context and how civil society continues to work towards positive change.

Five years after nation-wide protests in Algeria, Iraq, Lebanon, and Sudan demanded systemic changes and political reforms, the situation remains largely unchanged. In some cases, it has deteriorated. Sudan is facing a humanitarian catastrophe amidst an ongoing civil war. Lebanon is dealing with Israeli aggression amidst a severe economic crisis with little response from the caretaker government. In Iraq and Algeria, relative stability masks the reality of increased suppression of dissent. This preservation of the status quo supports the entrenched political structures that strive to uphold it.

The current absence of large-scale street protests in these countries should not be taken as an indication that populations are content with the status quo. The issues that ignited the initial uprisings remain and in many cases have worsened. Despite enormous challenges, activists continue to navigate their systems to survive and instigate change. In the face of increasing difficulties, they are raising awareness of their countries’ predicaments and are finding alternative economic solutions. Additionally they are mobilizing community support, and pushing to voice their disillusionment. All these efforts aim at actively participating in shaping decisions that determines their future.

This webinar explores:

• What has been the impact of the uprisings in Algeria, Iraq, Lebanon, and Sudan?
• How are civil society and activists contributing to change within their communities?
• What is the current landscape for civic engagement within the politics, society and economy in these countries?
• What prospects are there for solidarity and cooperation among civil society actors across these regions and beyond?

Gijs den Besten
Sep 1, 2013; 54:2325-2340
Reviews

Expert

Expert

A growing number of motor vehicle crashes across Jamaica has been attributed to drivers operating the vehicles under the influence of marijuana, according to Health Minister Dr Christopher Tufton. "The data is clear, we are seeing more people...

Reliable, specific polyclonal and monoclonal antibodies are important tools in research and medicine. However, the discovery of antibodies against their targets in their native forms is difficult. Here, we present a novel method for discovery of antibodies against membrane proteins in their native configuration in mammalian cells. The method involves the co-expression of an antibody library in a population of mammalian cells that express the target polypeptide within a natural membrane environment on the cell surface. Cells that secrete a single-chain fragment variable (scFv) that binds to the target membrane protein thereby become self-labeled, enabling enrichment and isolation by magnetic sorting and FRET-based flow sorting. Library sizes of up to 109 variants can be screened, thus allowing campaigns of naïve scFv libraries to be selected against membrane protein antigens in a Chinese hamster ovary cell system. We validate this method by screening a synthetic naïve human scFv library against Chinese hamster ovary cells expressing the oncogenic target epithelial cell adhesion molecule and identify a panel of three novel binders to this membrane protein, one with a dissociation constant (KD) as low as 0.8 nm. We further demonstrate that the identified antibodies have utility for killing epithelial cell adhesion molecule–positive cells when used as a targeting domain on chimeric antigen receptor T cells. Thus, we provide a new tool for identifying novel antibodies that act against membrane proteins, which could catalyze the discovery of new candidates for antibody-based therapies.

12 August 2020

20 August 2020

RAS genes are the most commonly mutated in human cancers and play critical roles in tumor initiation, progression, and drug resistance. Identification of targets that block RAS signaling is pivotal to develop therapies for RAS-related cancer. As RAS translocation to the plasma membrane (PM) is essential for its effective signal transduction, we devised a high-content screening assay to search for genes regulating KRAS membrane association. We found that the tyrosine phosphatase PTPN2 regulates the plasma membrane localization of KRAS. Knockdown of PTPN2 reduced the proliferation and promoted apoptosis in KRAS-dependent cancer cells, but not in KRAS-independent cells. Mechanistically, PTPN2 negatively regulates tyrosine phosphorylation of KRAS, which, in turn, affects the activation KRAS and its downstream signaling. Consistently, analysis of the TCGA database demonstrates that high expression of PTPN2 is significantly associated with poor prognosis of patients with KRAS-mutant pancreatic adenocarcinoma. These results indicate that PTPN2 is a key regulator of KRAS and may serve as a new target for therapy of KRAS-driven cancer.

Editing and/or saving an action column can take up to a minute to display a window. There are no workarounds identified at this time.

In SAS Retail Space Management, it should be possible to click on any location object, then Show Properties, and change the location fill color. This can be done on the gray-filled objects. However, w

Sphingolipids have emerged as bioactive lipids involved in the regulation of many physiological and pathological processes. In the retina, they have been established to participate in numerous processes, such as neuronal survival and death, proliferation and migration of neuronal and vascular cells, inflammation, and neovascularization. Dysregulation of sphingolipids is, therefore, crucial in the onset and progression of retinal diseases. This review examines the involvement of sphingolipids in retinal physiology and diseases. Ceramide (Cer) emerges as a common mediator of inflammation and death of neuronal and retinal pigment epithelium cells in animal models of retinopathies such as glaucoma, age-related macular degeneration (AMD), and retinitis pigmentosa. Sphingosine-1-phosphate (S1P) has opposite roles, preventing photoreceptor and ganglion cell degeneration but also promoting inflammation, fibrosis, and neovascularization in AMD, glaucoma, and pro-fibrotic disorders. Alterations in Cer, S1P, and ceramide-1-phosphate may also contribute to uveitis. Notably, use of inhibitors that either prevent Cer increase or modulate S1P signaling, such as Myriocin, desipramine, and Fingolimod (FTY720), preserves neuronal viability and retinal function. These findings underscore the relevance of alterations in the sphingolipid metabolic network in the etiology of multiple retinopathies and highlight the potential of modulating their metabolism for the design of novel therapeutic approaches.

Ankylosing Spondylitis (AS) is a common, inflammatory rheumatic disease, which primarily affects the axial skeleton and is associated with sacroiliitis, uveitis and enthesitis. Unlike other autoimmune rheumatic diseases, such as rheumatoid arthritis or systemic lupus erythematosus, autoantibodies have not yet been reported to be a feature of AS. We therefore wished to determine if plasma from patients with AS contained autoantibodies and if so, characterize and quantify this response in comparison to patients with Rheumatoid Arthritis (RA) and healthy controls. Two high-density nucleic acid programmable protein arrays expressing a total of 3498 proteins were screened with plasma from 25 patients with AS, 17 with RA and 25 healthy controls. Autoantigens identified were subjected to Ingenuity Pathway Analysis in order to determine patterns of signalling cascades or tissue origin. 44% of patients with Ankylosing Spondylitis demonstrated a broad autoantibody response, as compared to 33% of patients with RA and only 8% of healthy controls. Individuals with AS demonstrated autoantibody responses to shared autoantigens, and 60% of autoantigens identified in the AS cohort were restricted to that group. The AS patients autoantibody responses were targeted towards connective, skeletal and muscular tissue, unlike those of RA patients or healthy controls. Thus, patients with AS show evidence of systemic humoral autoimmunity and multispecific autoantibody production. Nucleic Acid Programmable Protein Arrays constitute a powerful tool to study autoimmune diseases.

The Phillies acquired All-Star catcher J.T. Realmuto from the Marlins on Thursday for catcher Jorge Alfaro, right-hander Sixto Sanchez, left-hander Will Stewart and $250,000 in international bonus slot money, the latest move in a busy and ambitious offseason for a team eager to return to the postseason.

Gabe Kapler is trying to keep the focus on his players in 2019, but it might be an impossible task.

Will Rhys Hoskins be bummed if Bryce Harper signs elsewhere?

MLB.com looked at each team's two highest-ranked position players in WAR, according to the Steamer projections. Here are the top 10, but keep in mind that things could change once Manny Machado and Bryce Harper find homes.

Oregon, Ohio State, Georgia and Miami lead the first edition of the 2024-25 College Football Playoff rankings, the selection committee announced.

Lionel Messi said Inter Miami will get ready to "come back stronger next year" after the Herons' upset loss to Atlanta United in the first round of the 2024 MLS Cup playoffs.