Gov. Eric Holcomb: "If you're 65 or older ... you're going to be living in a new normal for a while."

The film grossed only $33 million in its domestic debut.

Vin Diesel stars as Bloodshot as Valiant Comics finally makes it to the big screen.

Can you really blame voters, disillusioned and disappointed as they are, for tuning out the onslaught on American democracy?

oscon: Only 48 hours left to get discounted tickets to #OSCON. Early registration prices end tomorrow at midnight http://t.co/E0JKpcj1Rp

The following article, BREAKING: Michigan’s Radical Governor Extends Lockdown AGAIN…Only One Day After Church Leaders and MI Lawmakers File Suit Against Her, was first published on 100PercentFedUp.com.

Michigan was the first state to see protests against draconian lockdown measures by an overreaching governor. On April 15, the Michigan Conservative Coalition organized an in-vehicle protest in Lansing, MI that drew an estimated 15,000 vehicles. Since that time, several protests by Michigan workers who are being devastated by the one-size-fits-all shut down of the […]

Continue reading: BREAKING: Michigan’s Radical Governor Extends Lockdown AGAIN…Only One Day After Church Leaders and MI Lawmakers File Suit Against Her ...

Ontario’s provincial parks and conservation areas will reopen this week but campgrounds and beaches will continue to be off-limits for now.

Curtis Triplitt
Oct 1, 2006; 19:202-211
Pharmacy Update

Curtis Triplitt
Oct 1, 2006; 19:202-211
Pharmacy Update

Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the PGK1-catalyzed forward reaction. The Km values for 1,3-bisphosphoglyceric acid (1,3-BPG, the forward reaction substrate) were 4.36 μm (yeast PGK1) and 6.86 μm (human PKG1). The Km values for 3-phosphoglycerate (3-PG, the reverse reaction substrate and a serine precursor) were 146 μm (yeast PGK1) and 186 μm (human PGK1). The Vmax of the forward reaction was about 3.5- and 5.8-fold higher than that of the reverse reaction for the human and yeast enzymes, respectively. Consistently, the intracellular steady-state concentrations of 3-PG were between 180 and 550 μm in cancer cells, providing a basis for glycolysis to shuttle 3-PG to the serine synthesis pathway. Using siRNA-mediated PGK1-specific knockdown in five cancer cell lines derived from different tissues, along with titration of PGK1 in a cell-free glycolysis system, we found that the perturbation of PGK1 had no effect or only marginal effects on the glucose consumption and lactate generation. The PGK1 knockdown increased the concentrations of fructose 1,6-bisphosphate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, and 1,3-BPG in nearly equal proportions, controlled by the kinetic and thermodynamic states of glycolysis. We conclude that perturbation of PGK1 in cancer cells insignificantly affects the conversion of glucose to lactate in glycolysis.

Xia Meng
May 1, 2020; 61:591-591
Images in Lipid Research

Zinc metallopeptidase STE24 (ZMPSTE24) is essential for the conversion of farnesyl–prelamin A to mature lamin A, a key component of the nuclear lamina. In the absence of ZMPSTE24, farnesyl–prelamin A accumulates in the nucleus and exerts toxicity, causing a variety of disease phenotypes. By ~4 months of age, both male and female Zmpste24^–/– mice manifest a near-complete loss of adipose tissue, but it has never been clear whether this phenotype is a direct consequence of farnesyl–prelamin A toxicity in adipocytes. To address this question, we generated a conditional knockout Zmpste24 allele and used it to create adipocyte-specific Zmpste24–knockout mice. To boost farnesyl–prelamin A levels, we bred in the "prelamin A–only" Lmna allele. Gene expression, immunoblotting, and immunohistochemistry experiments revealed that adipose tissue in these mice had decreased Zmpste24 expression along with strikingly increased accumulation of prelamin A. In male mice, Zmpste24 deficiency in adipocytes was accompanied by modest changes in adipose stores (an 11% decrease in body weight, a 23% decrease in body fat mass, and significantly smaller gonadal and inguinal white adipose depots). No changes in adipose stores were detected in female mice, likely because prelamin A expression in adipose tissue is lower in female mice. Zmpste24 deficiency in adipocytes did not alter the number of macrophages in adipose tissue, nor did it alter plasma levels of glucose, triglycerides, or fatty acids. We conclude that ZMPSTE24 deficiency in adipocytes, and the accompanying accumulation of farnesyl–prelamin A, reduces adipose tissue stores, but only modestly and only in male mice.

Brendan McKay is still going to be a two-way player, but the Rays are planning to narrow his focus on the hitting side by keeping him to designated hitter-only duties, a source confirmed to MLB.com's Juan Toribio on Monday.

Katja Iversen, CEO of Women Deliver, joins Rebecca Coombes to explain why the UN sustainable development goals are unachievable if we don't empower women and girls to take control of their health, wellbeing, and reproductive rights. http://womendeliver.org/

A TB infection can take two forms, active and latent. Active disease is transmissible, and causes the damage to the lungs which makes TB one of the biggest killers in the world. In the latent form, the bacteria Mycobacterium tuberculosis is quiescent and can stay that way for years until it becomes active and causes those clinical signs. Testing...

Coronavirus brings worldwide horse racing almost to a halt, with only one Grade 1 event on the weekend schedule, but rays of hope exist.

Conservation officials in Ontario are asking members of the public to be on the lookout for a former pet snake that was mistakenly released into the wild near a conservation area.

Ricardo V. Cohen
Jul 1, 2012; 35:1420-1428
Diabetes Care Symposium

As a new index in the Web of Science™ Core Collection, Emerging Sources Citation Index (ESCI) expands the citation universe and reflects the growing global body of science and scholarly activity. Coverage is from 2015-

Canada Goose has long been a leader in the outdoor gear space.

Supai is so remote, mail is delivered by mule train

The pint-sized institution, which opened last year, is filled with 1,000 feline-themed knickknacks that journeyed with their owners from Ukraine

New research details the first forensic investigation of the Sylt concentration camp, located on the Channel Island of Alderney, since the end of WWII

Yukon officials say the territory's campgrounds will open to campers on June 4, but the territory's borders will remain closed. They also issued guidelines for some health care services to reopen.

The coronavirus pandemic has forced major professional sports leagues across North America to push the pause button. But even if the NHL, CFL, NBA or MLS are given the green light, many fans have reservations about returning to arenas until a vaccine for the virus is available.

The time was right for Lisa Weagle to join forces with Olympic champion Jennifer Jones. Jones told CBC Sports' Anastasia Bucsis that the latest addition to her five-person rink "was like the clouds parted and the sun came out."

In what are the lowest prices we've seen on Mid 2019 15-inch MacBook Pros, Amazon-owned Woot has refurbished units discounted to as low as $1,579.99, with savings of up to $950 off. These units are refurbished by Apple, but come with a 1-year Woot warranty.

When OM Montenegro began in 2007 with a team of three, holding a full Sunday meeting seemed a long way off—but not anymore.

Four OM guys in Bar, Montenegro, and four guys from a nearby Roma camp take a hiking/camping trip together.