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Chemical roadblocking of DNA transcription for nascent RNA display [RNA]

Site-specific arrest of RNA polymerases (RNAPs) is fundamental to several technologies that assess RNA structure and function. Current in vitro transcription “roadblocking” approaches inhibit transcription elongation by blocking RNAP with a protein bound to the DNA template. One limitation of protein-mediated transcription roadblocking is that it requires inclusion of a protein factor extrinsic to the minimal in vitro transcription reaction. In this work, we developed a chemical approach for halting transcription by Escherichia coli RNAP. We first established a sequence-independent method for site-specific incorporation of chemical lesions into dsDNA templates by sequential PCR and translesion synthesis. We then show that interrupting the transcribed DNA strand with an internal desthiobiotin-triethylene glycol modification or 1,N6-etheno-2'-deoxyadenosine base efficiently and stably halts Escherichia coli RNAP transcription. By encoding an intrinsic stall site within the template DNA, our chemical transcription roadblocking approach enables display of nascent RNA molecules from RNAP in a minimal in vitro transcription reaction.




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Julia Gillard on Breaking Barriers for Women in Politics

6 November 2019

Gitika Bhardwaj

Editor, Communications & Publishing, Chatham House

The Hon Julia Gillard AC

Prime Minister of Australia and Leader of the Australian Labor Party (2010-13)
In a series exploring women in international affairs, Julia Gillard speaks to Gitika Bhardwaj about serving in the highest political office in Australia and why she believes things are now changing for women around the world.

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Julia Gillard discusses her career as a woman in politics and why things are now changing for women in Australia and around the world. Photo: Chatham House.

Julia Gillard, you became the first female prime minister in Australian history in 2010, what have been the challenges and opportunities for you as a woman working in politics? Have the obstacles women face in positions of power changed over the years, and if so, how? 

I want to start positive and say I’m a huge advocate for people going into politics – particularly women. I believe there’s no better way of putting your values into action than going into politics but I’m not going to pretend that there’s no gender bit.

There still is a gender bit and I experienced that personally. A disproportionate focus on appearance, a disproportionate focus on family structures – for example the fact that I didn’t have kids – and the gendered insults becoming the go-to weapon when politics got turbulent, which inevitably happens, as governments make decisions that not everybody agrees with.

In terms of politics past and politics present, I think a lot has changed for the positive. There are more women in politics now which means more role models for other women. There’s been more of an attempt to have the system offer flexibilities for work and family life too. In Australian politics, famously, the non-members bar was replaced by a childcare centre, so that’s giving you a sense that there has been progress. We’ve also just hit the stage where our Senate is now 50 per cent men and 50 per cent women.

But I do think that there’s a new toxicity for women that’s been introduced through social media – through the fact that it’s anonymous and people can say anything and the kinds of revolting material many women politicians receive. I think that there’s a new coarseness in our traditional media too which means things will be said about people in politics today, especially women, which would not have been put in the pages of respectable newspapers 10 or 20 years ago.

So, it’s a mixed picture, where there has been major steps forward but there are still some huge issues to resolve.

Following this year’s elections, there are now a record number of female members of parliament in Australia, yet some argue that women are still underrepresented across the major political parties, and over the past 20 years, the country has fallen from 15th in the world to 50th for gender diversity in its parliament.

Given some of the recent experiences of women in Australian politics, do you think the major political parties are doing enough to address gender diversity in their ranks? 

I certainly think on the Labor side of politics important changes have happened in our political party and the benefit of those changes has showed. I’m of that generation of Labor women that fought for an affirmative action target and we had that adopted as a Labor party rule in the early 1990s. It started at 30 per cent, and it’s gone up over time, and the benefit of that now is that the Labor Party is almost at 50-50 per cent men and women, coming off a very low base in the early 1990s where we were at 14 per cent men and women. 

The Conservative side for politics hasn’t embraced a target or quota as of yet. They have done some things, through mentoring and networking and training, but that hasn’t seen as significant a shift in the gender diversity in their ranks. They’ve moved slowly from when they were 13 per cent women to now where they’re in the mid-20 per cent. 

Of course that doesn’t mean the work within Labor is done: we’ve got to keep delivering to the affirmative action target, having women come through for all of the ministries, the Cabinet and for all of the portfolios and to make sure that we’re embracing the full diversity of women too. Australia is a very multicultural society and there is more to do to make sure that women – and men – in the Australian parliament represents that diversity. 

During your premiership, you delivered a famous speech on misogyny and sexism and described there being 'gender wars' in Australian politics. How far has Australia addressed its problems regarding everything from unconscious bias to gender stereotypes? Do you think social attitudes in Australia to women in leadership are changing?

I don’t think these issues are particularly an Australian problem. When I left politics, people kept asking me about my experiences and it became convenient for them to say ‘That’s Australia and its macho culture and Crocodile Dundee and all of that.’ I was always quick to point out, actually, a number of the insults hurled at me were first hurled at Hillary Clinton when she originally put her name forward to be considered as a candidate for US president. So this is not an Australian problem – it’s a global problem. 

I can see progress in Australia though. When I was prime minister, the sort of fashionable analysis by the press was that nothing, in my experience, had anything to do with gender – I was just being treated like every prime minister had always been treated.

Today, there is a very lively debate about sexism in Australian politics and about how women can feel excluded from these structures with various conservative women making complaints about bullying within their political party. So the preparedness to report issues due to the understanding of gender is now much higher and I’m a big believer that you never solve a problem unless you start talking about it so I’m glad we’re talking about it now.

From the implementation of ‘womenomics’ in Japan, to gender-responsive budgeting in Indonesia, countries around the world are making progress towards addressing gender issues, yet, structural and cultural barriers that prevent women’s economic, political and social participation remain.

What are the biggest barriers that women face around the world and do you think enough is being done to address these barriers?

I think so much is context-specific that it’s hard to say, but I would say, in some parts of the world, unequal access to education is the fundamental barrier.

Now that’s not true in the UK or in Australia, where the statistics tell you that disproportionately graduates today are women and not men, but if we look at many of the poorer parts of the world, like in sub-Saharan Africa and other places, there are 260 million children out of school – and the face of a child most likely to miss out is a female face. So there still needs to be a lot more progress on things such as equal access to education around the world. 

In many parts of the developed world, there is actually an assault now on long-held rights around women’s reproduction so I think that is another foundation stone – and then really it comes to a set of issues and barriers around the world of work and full access to every level of work.

Much of this is what we research at the Global Institute for Women’s Leadership because we continue to see workplaces and organizations that have a very traditional view of what merit looks like. It’s a sort of male-defined view of the world and it is one that is not inclusive of women. We are still seeing the unequal sharing of domestic labour which has ramifications for women’s engagement in the world of work and their ability to achieve leadership within it too. 

With a broad brush, I would point to all of this, but the most pressing problems that women continue to face varies from place to place.

Julia Gillard speaking at the House of Representatives on 5 February 2013 in Canberra, Australia. Photo: Getty Images.

Globally, increasing numbers of women are being elected to political office, from the first female president of Slovakia to the first female mayor of Tunis. This comes at a time of record numbers of female ministers in Egypt and Jordan as well as gender-balanced cabinets in 10 countries worldwide – six of which were achieved in 2018 alone.

Do you think women’s rights and gender equality are benefitting from more female representation in politics and how are female voters responding, if at all, to this increase in the number of women holding political office?

I think, even if women didn’t bring new policy perspectives to the world of politics, I would still be an advocate of gender equality in politics because I believe merit is equally distributed between the sexes.

If you see women being represented at less than 50 per cent then that’s got to mean that there are women of merit who didn’t get there – who should’ve gotten there. I think it’s important to make that point otherwise we’re saying ‘Women should only be there if, when they are there, they do this, this and this’. We don’t tend to put that ‘if’ in sentences about men.

But I do think the evidence shows that more women, being involved in politics, does diversify the public policy agenda. That doesn’t mean that a male politician couldn’t focus his career and advocacy on childcare or domestic violence or combating sexual assault or furthering women’s reproductive rights. But I think the evidence shows that there is a lived experience that women bring to politics that enables them to mobilize around a set of issues that are of particular concern to women.

On the role model effect, I think the evidence shows that, if women and girls do see role models, they are more likely to think that that is a pathway open to them. One of the things that does slightly concern me is whether that evidence is now retrospective evidence and whether the prospective evidence is going to be – because of the toxicity of social media – more women thinking about the real-world threats that being in politics presents for them. And so the role modelling effect will work in reverse because it will show how women are treated in politics is more of a negative than a positive.

I certainly hope this doesn’t happen and young women are encouraged to go into politics. That’s where we still have to shine a light on the positive aspects of what working in politics has to offer.

Despite all of the progress we are seeing, women are still faced with gender-based discrimination and gender-based violence, virtually and physically, with 40 per cent of women and girls living in countries which fail to guarantee basic standards of gender equality. What do you think needs to happen to ultimately realize women’s rights and gender equality globally? Are you hopeful this will be achieved in the future?

Yes I’m an optimist overall. People like to quote the great Martin Luther King quote ‘The moral arc of the universe bends towards justice.’ I believe that but I think sometimes the imagery of the arc as if it’s always in a forward movement hides the nitty-gritty struggle that is there beneath. 

Inevitably, at some points, it feels like there’s more of a backlash against women than a forward movement. But, over time, I’m an optimist that the forward movement wins through. 

I do believe we can reach a stage where societies are generating societies where women can live their lives free from the threat of sexual violence or discriminatory treatment based on gender.




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Network Power in the Asia-Pacific: Making Sense of the New Regionalism and Opportunities for Cooperation

Research Event

7 February 2020 - 9:45am to 5:30pm

Chatham House | 10 St James's Square | London | SW1Y 4LE

The Asia-Pacific region continues to increase in geopolitical and geoeconomic importance. The rise of China and tensions with the US are affecting bilateral relationships and traditional alliances in the region. Whether seen from the perspective of the Quad – Australia, India, Japan and the US – or the Indo-Pacific concept embraced by a wide range of countries but with no shared consensus on scope and objectives or with ASEAN who insists on the importance of its own centrality, the region is redefining and reconceptualising itself.

With a diverse range of initiatives – including the Regional Comprehensive Economic Partnership (RCEP) and Comprehensive and Progressive Agreement for Trans-Pacific Partnership (CPTPP) – there are a plethora of regional agreements and institutional groupings that add further complexity.

As the Bretton Woods architecture continues to be dominated by Western powers, China is also spearheading parallel governance initiatives such as the Asian Infrastructure Investment Bank (AIIB), the Belt and Road Initiative and the Shanghai Cooperation Organization (SCO) as a means of enhancing its geopolitical and geoeconomic influence.

This one-day conference will focus on how such networks and alliances have been built, and sustained, in the Asia-Pacific region. In order to understand how new regional initiatives might open up opportunities for new forms of international cooperation, the conference will focus on the themes of cyber-technology and innovation, sustainable development and mitigating the impacts of climate change and new infrastructure initiatives. It will assess whether there is a zero-sum conflict between competing networks and agendas or whether a common approach can be developed.

Lucy Ridout

Programme Administrator, Asia-Pacific Programme
+44 (0) 207 314 2761




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Making Movies Come Alive

Many movie animation techniques are based on mathematics. Characters, background, and motion are all created using software that combines pixels into geometric shapes which are stored and manipulated using the mathematics of computer graphics. Software encodes features that are important to the eye, like position, motion, color, and texture, into each pixel. The software uses vectors, matrices, and polygonal approximations to curved surfaces to determine the shade of each pixel. Each frame in a computer-generated film has over two million pixels and can have over forty million polygons. The tremendous number of calculations involved makes computers necessary, but without mathematics the computers wouldn.t know what to calculate. Said one animator, ". . . it.s all controlled by math . . . all those little X,Y.s, and Z.s that you had in school - oh my gosh, suddenly they all apply." For More Information: Mathematics for Computer Graphics Applications, Michael E. Mortenson, 1999.




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Making Votes Count

The outcome of elections that offer more than two alternatives but with no preference by a majority, is determined more by the voting procedure used than by the votes themselves. Mathematicians have shown that in such elections, illogical results are more likely than not. For example, the majority of this group want to go to a warm place, but the South Pole is the group.s plurality winner. So if these people choose their group.s vacation destination in the same way most elections are conducted, they will all go to the South Pole and six people will be disappointed, if not frostbitten. Elections in which only the top preference of each voter is counted are equivalent to a school choosing its best student based only on the number of A.s earned. The inequity of such a situation has led to the development of other voting methods. In one method, points are assigned to choices, just as they are to grades. Using this procedure, these people will vacation in a warm place a more desirable conclusion for the group. Mathematicians study voting methods in hopes of finding equitable procedures, so that no one is unfairly left out in the cold. For more information: Chaotic Elections: A Mathematician Looks at Voting, Donald Saari




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Working It Out. Math solves a mystery about the opening of "A Hard Day's Night."

The music of most hit songs is pretty well known, but sometimes there are mysteries. One question that remained unanswered for over forty years is: What instrumentation and notes make up the opening chord of the Beatles. "A Hard Day.s Night"? Mathematician Jason Brown - a big Beatles fan - recently solved the puzzle using his musical knowledge and discrete Fourier transforms, mathematical transformations that help decompose signals into their basic parts. These transformations simplify applications ranging from signal processing to multiplying large numbers, so that a researcher doesn.t have to be "working like a dog" to get an answer. Brown is also using mathematics, specifically graph theory, to discover who wrote "In My Life," which both Lennon and McCartney claimed to have written. In his graphs, chords are represented by points that are connected when one chord immediately follows another. When all songs with known authorship are diagrammed, Brown will see which collection of graphs - McCartney.s or Lennon.s - is a better fit for "In My Life." Although it may seem a bit counterintuitive to use mathematics to learn more about a revolutionary band, these analytical methods identify and uncover compositional principles inherent in some of the best Beatles. music. Thus it.s completely natural and rewarding to apply mathematics to the Fab 4 For More Information: Professor Uses Mathematics to Decode Beatles Tunes, "The Wall Street Journal", January 30, 2009..




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Working Up a Lather : Part 1

James Sethian and Frank Morgan talk about their research investigating bubbles.




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Thinking Outside the Box Score - Math and basketball: Part 1

Muthu Alagappan explains how topology and analytics are bringing a new look to basketball.




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Making an Attitude Adjustment: Part 1

Nazareth Bedrossian talks about using math to reposition the International Space Station.




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Working With the System: Part 2

Researcher: Cristina Stoica, Wilfrid Laurier University
Description: Cristina Stoica talks about celestial mechanics.




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Making Art Work

Researcher: Annalisa Crannell, Franklin & Marshall College. Annalisa Crannell on perspective in art.




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Scoring with New Thinking

Researcher: Andy Andres, Boston University Moment: http://www.ams.org/samplings/mathmoments/mm136-baseball.pdf Andy Andres on baseball analytics.




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Making the Earth Flat

Tom Patterson and Bojan Savric discuss the Equal Earth projection map that they created with Bernhard Jenny.




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Making Beautiful Mathematics

Rob Schneiderman talks about the metaphorical connections between math and music




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Unmasking Deepfakes

Hany Farid talks about fighting fake videos: "Mathematically, there's a lot of linear algebra, multivariate calculus, probability and statistics, and then a lot of techniques from pattern recognition, signal processing, and image processing."




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Looking for someone to create a Medium article




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Looking To Exchange Website Traffic For Memes Site




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Launch of the Website of the Online Survey on the Application of and Experience in the Use of Socio-Economic Considerations in Decision-Making on Living Modified Organisms




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Biosafety Protocol News Issue 8 - Working towards a common goal: Ten years of international cooperation on implementation of the Cartagena Protocol on Biosafety




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Two new biosafety self-paced learning modules on public access to biosafety information and public participation in decision-making regarding LMOs are available in the new e-Learning Platform




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CBD News: COP 9 Presidency pays Working Visit to the UNCBD Secretariat.




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CBD News: Statement by the Executive Secretary, Dr. Ahmed Djoghlaf, on the occasion of the First Meeting of the Ad Hoc Technical Expert Group on Biodiversity and Climate Change, London, United Kingdom , 17 - 21 November 2008.




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CBD News: Press Release - High-level Working Group on the Future of a Global Policy for Biodiversity and Ecosystems.




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CBD News: Statement by Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, at the Opening Session of the Seventh Meeting of the Ad Hoc Open-Ended Working Group on Access and Benefit-Sharing, UNESCO Headquarters, Paris, 2 Apr




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CBD News: Statement by Mr Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity on the occasion of the G8 Environment Ministers Meeting Opening Working Session on Biodiversity, 23 April 2009, Syracuse, Italy.




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CBD News: Sixth Trondheim Conference on Biodiversity on "Getting the biodiversity targets right - working for sustainable development", Trondheim, Norway, 1 - 5 February 2010




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CBD Notification: Expert consultation on the revision and updating of the Strategic Plan of the Convention, 18 - 20 January 2010, London, the United Kingdom.




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CBD News: Statement by Mr Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, at the Opening Session of the Sixth Meeting of the Ad Hoc Open-Ended Working Group on Article 8(j) and Related Provisions of the Convention on Biolog




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CBD News: Statement by Mr Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, at the closing session of the Sixth Meeting of the Ad Hoc Open-Ended Working Group on Article 8(j) and Related Provisions of the Convention on Biologi




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CBD News: Statement by Mr. Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, at the Opening Session of the Eighth Meeting of the Ad Hoc Open-Ended Working Group on Access and Benefit-Sharing, Montreal, 9 November 2009.




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CBD News: Statement by Mr. Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, at the Closing Session of the Eighth Meeting of the Ad Hoc Open-Ended Working Group on Access and Benefit-Sharing, Montreal, 15 November 2009.




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CBD Communiqué: CBD Secretariat and the Institute of Energy and Environment of La Francophonie join Forces to Raise Awareness in French-Speaking Countries.




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CBD News: The Natural History Museum (United Kingdom) launches its official International Year of Biodiversity website: www.biodiversityislife.net

http://www.biodiversityislife.net/




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CBD News: Statement on Behalf of Mr. Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, on the occasion of the Ad Hoc Open-Ended Informal Working Group to Study Issues Relating to the Conservation and Sustainable Use of Marine




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CBD Communiqué: CBD Secretariat and Conservation International Working to Build Strong Collaboration for Implementation of the CBD




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CBD News: Statement by Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, on the occasion of the Ninth Meeting of the Ad Hoc Open-Ended Working Group on Access and Benefit-Sharing, Cali, Colombia, 22 March 2010




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CBD News: Statement by Mr. Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, at the occasion of the Third Meeting of the Working Group on the Review of Implementation of the Convention on Biological Diversity (WGRI 3), Nairobi




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CBD News: Statement by Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, on the occasion of the Celebration of the International Year of Biodiversity by the Kingdom of Cambodia, Banteay Kdei Temple, Siem Reap Province, Cambodi




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CBD Notification: Resumed ninth meeting of the Ad Hoc Open-ended Working Group on Access and Benefit-sharing (WG ABS 9), Nagoya, Japan, 16 October 2010




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CBD News: Statement by Mr Ahmed Djoghlaf, CBD Executive Secretary, on the occasion of the Meeting of the Intergovernmental Technical Working Group on Forest Genetic Resources, towards the Report on the State of the world's forest genetic resources, 4-




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CBD News: Statement on Behalf of the Executive Secretary to the Convention on Biological Diversity on the occasion of Third Meeting of the Intersessional Working Group on Ocean Fertilization, 31 May - 3 June 2011




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CBD Communiqué: Expanding scientific expertise for implementation of the Strategic Plan for Biodiversity 2011-2020 at the margins of the seventh meeting of Working Group on Article 8(j) and the fifteenth meeting of SBSTTA




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CBD Press Release: Capacity-building workshop for North Africa and the Middle East on mainstreaming the economics of ecosystems and biodiversity (TEEB) into national planning and decision-making




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CBD News: Statement by the CBD Executive Secretary on the occasion of the Third Meeting of the Ad Hoc Working Group of the Whole on the Regular Process for Global Reporting and Assessment of the State of the Marine Environment, including Socio-economic As




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CBD News: Statement by the CBD Executive Secretary on the occasion of the fifth meeting of the Ad Hoc Open-ended Informal Working Group to study issues relating to the conservation and sustainable use of marine biological diversity beyond areas of nationa




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CBD News: Statement by Mr. Braulio Ferreira de Souza Dias, CBD Executive Secretary, on the occasion of the fourth meeting of the Ad Hoc Working Group on the Review of Implementation of the Convention, Montreal, Canada, 7 May 2012




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CBD News: The High-Level Panel (HLP), co-sponsored by the Governments of India and the United Kingdom, has organized a one-day open consultation with Parties. The Webinar will take place on 2 August 2012 from 11:00 a.m. to 5:00 p.m. (U.K. time)




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CBD News: At COP 11, the CBD Executive Secretary has invited Parties and partners to become Biodiversity Champions by making pledges to suport the achievement of the Aichi Biodiversity Targets.




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CBD News: As we remember her, let us be inspired by her tireless energy, by her commitment to making a better world for all and by her leadership and vision.




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CBD News: Statement by Mr. Braulio F. de Souza Dias, CBD Executive Secretary, to the International Seminar "Towards Linking Ecosystems and Ecosystem Services to Economic and Human Activity", New York, United States of America, 27 - 29 November 2