As of today, the total number of confirmed COVID-19 cases in India is 59662, with 17847 recovered cases and 1981 deaths. The concerns related to COVID-19 pandemic is increasing day by day. The WHO and CDC along with many healthcare institutions

The party said Parliament should assert itself and not allow this to happen, as it called upon the Centre and the states to jointly formulate an action plan to ensure safe return of migrants to their homes.

Actress Shriya Saran is leaving no stone unturned to use her stardom amid lockdown. Well, the star, who is currently home quarantined in Spain with husband Andrei Koscheev, is doing everything possible to raise funds for COVID-19 relief. And

At least eight people, including a child, died today morning after a toxic gas leaked from a chemical plant of a company in Visakhapatnam, Andhra Pradesh. Fire engines, police and ambulances have reached the area to control the situation and around

Each and every minute detail of Allu Arjun's Pushpa has left the fans super excited, ever since the inception. The movie is said to be an action thriller, wherein Allu Arjun will be seen doing some high-octane action sequences. Well, if

Growing a beard has now become a fashion amongst youngsters. Nowadays, a majority of girls love to see their partners with a beard. Cinema is the main reason for making this beard look famous amongst the masses. South Industries

A few days ago, Vijay Deverakonda expressed his anger against websites publishing fake news about him. For those who are unversed, in a recent video, angry Vijay revealed that some websites spread fake news against his charitable trust, which gained all

For context, the nationwide lockdown is scheduled to end on May 17 but Centre has allowed reopening of industries in non-hotspot areas with some conditions.

Lewis Library - ML172.H4 2019

Lewis Library - ML172.D47 2018

Online Resource

Hayden Library - TA418.9.N35 F87 2018

Production from PSCs now stands around 30% share of domestic oil, 20% of natural gas

While airlines are not keen on leaving a seat vacant in between rows to ensure social distancing, they are readying alternative plans for starting pos

The number of active cases of coronavirus in Uttar Pradesh rose to 1,884 on Sunday, while 1,504 patients have been discharged so far, a senior official said. Principal secretary, health, Amit Mohan Prasad said 84 more people have tested positive for the disease.

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1. UFC 249: Reaction to Justin Gaethje’s TKO victory over Tony Ferguson  NEWS.com.au
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3. Dana White reacts to epic Justin Gaethje vs. Tony Ferguson war | UFC 249 Post-Fight Press Conference  TheMacLife
4. UFC 249: Justin Gaethje, Tony Ferguson result, Henry Cejudo vs Dominik Cruz  NEWS.com.au
5. UFC 249 gets Mixed Martial Arts back underway behind closed doors in Florida  ABC News
6. View Full coverage on Google News

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2. Disney+'s Prop Culture scores rare Rick Moranis interview  The A.V. Club
3. Rick Moranis makes rare appearance after turning his back on movie career  New Zealand Herald
4. View Full coverage on Google News

In a step that brings silicon-based quantum computers closer to reality, researchers at Princeton University have built a device in which a single electron can pass its quantum information to a particle of light. The particle of light, or photon, can then act as a messenger to carry the information to other electrons, creating connections that form the circuits of a quantum computer.

Princeton University researchers have discovered that the bacteria behind the life-threatening disease cholera initiates infection by coordinating a wave of mass shapeshifting that allows them to more effectively penetrate their victims' intestines. The researchers also identified the protein that allows Vibrio cholerae to morph, and found that it's activated through quorum sensing. The findings could lead to new treatments for cholera that target the bacteria's ability to change shape or penetrate the gut.

For Immediate Release No. 20-OPA-042

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He's still giving the money back though.

He's still giving the money back though.

He's still giving the money back though.

The gloves — and filters — are off.

When a famed Egyptian actor revealed his daughter had transitioned, the reaction was unconventional.

The BBC speaks to unexpected sources who are supplying PPE amid coronavirus shortages.

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The post REPORT: Actor says ‘rule of law is dead’ until Barr is ‘impeached’ appeared first on Dennis Michael Lynch.

Research Event

Event participants

Dr Tim Summers, Senior Consulting Fellow, Asia Programme, Chatham House

Invitation Only Research Event

Event participants

Dr Leena Koni Hoffmann, Centre for Population, Poverty and Public Policy Studies; Author, Who Speaks for the North? Politics and Influence in Northern Nigeria; ERANDA Junior Research Fellow, Africa Programme, Chatham House (2013)
Chair: Elizabeth Donnelly, Assistant Head, Africa Programme, Chatham House

Research Event

Event participants

Christopher K Johnson, Senior Adviser; Freeman Chair in China Studies, Center for Strategic and International Studies
Chair: Dr Michal Meidan, Associate Fellow, Asia Programme, Chatham House 

In a tweet Saturday morning, Tesla's chief executive said it would file a lawsuit against county officials over not being able to run its factory.

Bacterial products such as lipopolysaccharides (or endotoxin) cause systemic inflammation, resulting in a substantial global health burden. The onset, progression, and resolution of the inflammatory response to endotoxin are usually tightly controlled to avoid chronic inflammation. Members of the NF-κB family of transcription factors are key drivers of inflammation that activate sets of genes in response to inflammatory signals. Such responses are typically short-lived and can be suppressed by proteins that act post-translationally, such as the SOCS (suppressor of cytokine signaling) family. Less is known about direct transcriptional regulation of these responses, however. Here, using a combination of in vitro approaches and in vivo animal models, we show that endotoxin treatment induced expression of the well-characterized transcriptional repressor Krüppel-like factor 3 (KLF3), which, in turn, directly repressed the expression of the NF-κB family member RELA/p65. We also observed that KLF3-deficient mice were hypersensitive to endotoxin and exhibited elevated levels of circulating Ly6C+ monocytes and macrophage-derived inflammatory cytokines. These findings reveal that KLF3 is a fundamental suppressor that operates as a feedback inhibitor of RELA/p65 and may be important in facilitating the resolution of inflammation.

Protease-activated receptor 2 (PAR-2) is activated by secreted proteases from immune cells or fungi. PAR-2 is normally expressed basolaterally in differentiated nasal ciliated cells. We hypothesized that epithelial remodeling during diseases characterized by cilial loss and squamous metaplasia may alter PAR-2 polarization. Here, using a fluorescent arrestin assay, we confirmed that the common fungal airway pathogen Aspergillus fumigatus activates heterologously-expressed PAR-2. Endogenous PAR-2 activation in submerged airway RPMI 2650 or NCI–H520 squamous cells increased intracellular calcium levels and granulocyte macrophage–colony-stimulating factor, tumor necrosis factor α, and interleukin (IL)-6 secretion. RPMI 2650 cells cultured at an air–liquid interface (ALI) responded to apically or basolaterally applied PAR-2 agonists. However, well-differentiated primary nasal epithelial ALIs responded only to basolateral PAR-2 stimulation, indicated by calcium elevation, increased cilia beat frequency, and increased fluid and cytokine secretion. We exposed primary cells to disease-related modifiers that alter epithelial morphology, including IL-13, cigarette smoke condensate, and retinoic acid deficiency, at concentrations and times that altered epithelial morphology without causing breakdown of the epithelial barrier to model early disease states. These altered primary cultures responded to both apical and basolateral PAR-2 stimulation. Imaging nasal polyps and control middle turbinate explants, we found that nasal polyps, but not turbinates, exhibit apical calcium responses to PAR-2 stimulation. However, isolated ciliated cells from both polyps and turbinates maintained basolateral PAR-2 polarization, suggesting that the calcium responses originated from nonciliated cells. Altered PAR-2 polarization in disease-remodeled epithelia may enhance apical responses and increase sensitivity to inhaled proteases.

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Inter-α-inhibitor is a proteoglycan essential for mammalian reproduction and also plays a less well-characterized role in inflammation. It comprises two homologous “heavy chains” (HC1 and HC2) covalently attached to chondroitin sulfate on the bikunin core protein. Before ovulation, HCs are transferred onto the polysaccharide hyaluronan (HA) to form covalent HC·HA complexes, thereby stabilizing an extracellular matrix around the oocyte required for fertilization. Additionally, such complexes form during inflammatory processes and mediate leukocyte adhesion in the synovial fluids of arthritis patients and protect against sepsis. Here using X-ray crystallography, we show that human HC1 has a structure similar to integrin β-chains, with a von Willebrand factor A domain containing a functional metal ion-dependent adhesion site (MIDAS) and an associated hybrid domain. A comparison of the WT protein and a variant with an impaired MIDAS (but otherwise structurally identical) by small-angle X-ray scattering and analytical ultracentrifugation revealed that HC1 self-associates in a cation-dependent manner, providing a mechanism for HC·HA cross-linking and matrix stabilization. Surprisingly, unlike integrins, HC1 interacted with RGD-containing ligands, such as fibronectin, vitronectin, and the latency-associated peptides of transforming growth factor β, in a MIDAS/cation-independent manner. However, HC1 utilizes its MIDAS motif to bind to and inhibit the cleavage of complement C3, and small-angle X-ray scattering–based modeling indicates that this occurs through the inhibition of the alternative pathway C3 convertase. These findings provide detailed structural and functional insights into HC1 as a regulator of innate immunity and further elucidate the role of HC·HA complexes in inflammation and ovulation.

β-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form β-cholesterylglucoside (β-GlcChol) in vitro. β-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate β-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (β-GalChol), in addition to β-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for β-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for β-GalChol formation. Liquid chromatography–tandem MS revealed that β-GlcChol and β-GalChol are present throughout development from embryo to adult in the mouse brain. We found that β-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of β-GalChol biosynthesis appeared to be during myelination. We also found that β-GlcChol and β-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain.

Actinobacillus pleuropneumoniae (App) is the etiological agent of acute porcine pneumonia and responsible for severe economic losses worldwide. The capsule polymer of App serotype 1 (App1) consists of [4)-GlcNAc-β(1,6)-Gal-α-1-(PO4-] repeating units that are O-acetylated at O-6 of the GlcNAc. It is a major virulence factor and was used in previous studies in the successful generation of an experimental glycoconjugate vaccine. However, the application of glycoconjugate vaccines in the animal health sector is limited, presumably because of the high costs associated with harvesting the polymer from pathogen culture. Consequently, here we exploited the capsule polymerase Cps1B of App1 as an in vitro synthesis tool and an alternative for capsule polymer provision. Cps1B consists of two catalytic domains, as well as a domain rich in tetratricopeptide repeats (TPRs). We compared the elongation mechanism of Cps1B with that of a ΔTPR truncation (Cps1B-ΔTPR). Interestingly, the product profiles displayed by Cps1B suggested processive elongation of the nascent polymer, whereas Cps1B-ΔTPR appeared to work in a more distributive manner. The dispersity of the synthesized products could be reduced by generating single-action transferases and immobilizing them on individual columns, separating the two catalytic activities. Furthermore, we identified the O-acetyltransferase Cps1D of App1 and used it to modify the polymers produced by Cps1B. Two-dimensional NMR analyses of the products revealed O-acetylation levels identical to those of polymer harvested from App1 culture supernatants. In conclusion, we have established a protocol for the pathogen-free in vitro synthesis of tailored, nature-identical App1 capsule polymers.