macOS and iOS have a vulnerability with ImageIO where memory safety issues occur when processing OpenEXR images.

Presentation by Jason Fullerton of ACI Technologies, Inc.

The FDIC is clarifying its policy and supervisory approach related to facilitating payment processing services directly, or indirectly through a third party, for merchant customers engaged in higher-risk activities.

The European Data Protection Board (EDPB) has published a set of guidelines (in draft) for public consultation. These will be absolutely key to providers of online services, such as social media, e-commerce, internet search engines, communication an...

The legal issues surrounding the processing of personal data relating to criminal convictions, allegations and offences or related security measures (“Criminal Records Data”) are complex. Education institutions tackle such issues on a re...

[ANGOP] Tômbwa -The Namibe provincial vice-governor for Political, Economic and Social Sector, Maiza Tavares advised this Friday in the municipality of Tômbwa the women who work on fish processing to improve the hygienic and sanitary condition in work places.

Approved project 53313-001 in Indonesia.

Dysregulation of DNA methylation is an established feature of breast cancers. DNA demethylating therapies like decitabine are proposed for the treatment of triple-negative breast cancers (TNBC) and indicators of response need to be identified. For this purpose, we characterized the effects of decitabine in a panel of 10 breast cancer cell lines and observed a range of sensitivity to decitabine that was not subtype specific. Knockdown of potential key effectors demonstrated the requirement of deoxycytidine kinase (DCK) for decitabine response in breast cancer cells. In treatment-naïve breast tumors, DCK was higher in TNBCs, and DCK levels were sustained or increased post chemotherapy treatment. This suggests that limited DCK levels will not be a barrier to response in patients with TNBC treated with decitabine as a second-line treatment or in a clinical trial. Methylome analysis revealed that genome-wide, region-specific, tumor suppressor gene–specific methylation, and decitabine-induced demethylation did not predict response to decitabine. Gene set enrichment analysis of transcriptome data demonstrated that decitabine induced genes within apoptosis, cell cycle, stress, and immune pathways. Induced genes included those characterized by the viral mimicry response; however, knockdown of key effectors of the pathway did not affect decitabine sensitivity suggesting that breast cancer growth suppression by decitabine is independent of viral mimicry. Finally, taxol-resistant breast cancer cells expressing high levels of multidrug resistance transporter ABCB1 remained sensitive to decitabine, suggesting that the drug could be used as second-line treatment for chemoresistant patients.

Activation of microglia and astrocytes secondary to inflammatory processes contributes to the development and perpetuation of pain with a neuropathic phenotype. This pain state presents as a chronic debilitating condition and affects a large population of patients with conditions like rheumatoid arthritis and diabetes, or after surgery, trauma, or chemotherapy. Here, we review the regulation of lipid rafts in glial cells and the role they play as a key component of neuroinflammatory sensitization of central pain signaling pathways. In this context, we introduce the concept of an inflammaraft (i-raft), enlarged lipid rafts harboring activated receptors and adaptor molecules and serving as an organizing platform to initiate inflammatory signaling and the cellular response. Characteristics of the inflammaraft include increased relative abundance of lipid rafts in inflammatory cells, increased content of cholesterol per raft, and increased levels of inflammatory receptors, such as toll-like receptor (TLR)4, adaptor molecules, ion channels, and enzymes in lipid rafts. This inflammaraft motif serves an important role in the membrane assembly of protein complexes, for example, TLR4 dimerization. Operating within this framework, we demonstrate the involvement of inflammatory receptors, redox molecules, and ion channels in the inflammaraft formation and the regulation of cholesterol and sphingolipid metabolism in the inflammaraft maintenance and disruption. Strategies for targeting inflammarafts, without affecting the integrity of lipid rafts in noninflammatory cells, may lead to developing novel therapies for neuropathic pain states and other neuroinflammatory conditions.

ABSTRACT

The posttranslational Ca²⁺-dependent "clip-and-link" activity of large repeat-in-toxin (RTX) proteins starts by Ca²⁺-dependent structural rearrangement of a highly conserved self-processing module (SPM). Subsequently, an internal aspartate-proline (Asp-Pro) peptide bond at the N-terminal end of SPM breaks, and the liberated C-terminal aspartyl residue can react with a free -amino group of an adjacent lysine residue to form a new isopeptide bond. Here, we report a solution structure of the calcium-loaded SPM (Ca-SPM) derived from the FrpC protein of Neisseria meningitidis. The Ca-SPM structure defines a unique protein architecture and provides structural insight into the autocatalytic cleavage of the Asp-Pro peptide bond through a "twisted-amide" activation. Furthermore, in-frame deletion of the SPM domain from the ApxIVA protein of Actinobacillus pleuropneumoniae attenuated the virulence of this porcine pathogen in a pig respiratory challenge model. We hypothesize that the Ca²⁺-dependent clip-and-link activity represents an unconventional strategy for Gram-negative pathogens to adhere to the host target cell surface.

IMPORTANCE The Ca²⁺-dependent clip-and-link activity of large repeat-in-toxin (RTX) proteins is an exceptional posttranslational process in which an internal domain called a self-processing module (SPM) mediates Ca²⁺-dependent processing of a highly specific aspartate-proline (Asp-Pro) peptide bond and covalent linkage of the released aspartyl to an adjacent lysine residue through an isopeptide bond. Here, we report the solution structures of the Ca²⁺-loaded SPM (Ca-SPM) defining the mechanism of the autocatalytic cleavage of the Asp414-Pro415 peptide bond of the Neisseria meningitidis FrpC exoprotein. Moreover, deletion of the SPM domain in the ApxIVA protein, the FrpC homolog of Actinobacillus pleuropneumoniae, resulted in attenuation of virulence of the bacterium in a pig infection model, indicating that the Ca²⁺-dependent clip-and-link activity plays a role in the virulence of Gram-negative pathogens.

ABSTRACT

Staphylococcus aureus can colonize the human host and cause a variety of superficial and invasive infections. The success of S. aureus as a pathogen derives from its ability to modulate its virulence through the release, sensing of and response to cyclic signaling peptides. Here we provide, for the first time, evidence that S. aureus processes and secretes small linear peptides through a specialized pathway that converts a lipoprotein leader into an extracellular peptide signal. We have identified and confirmed the machinery for each step and demonstrate that the putative membrane metalloprotease Eep and the EcsAB transporter are required to complete the processing and secretion of the peptides. In addition, we have identified several linear peptides, including the interspecies signaling molecule staph-cAM373, that are dependent on this processing and secretion pathway. These findings are particularly important because multiple Gram-positive bacteria rely on small linear peptides to control bacterial gene expression and virulence.

IMPORTANCE Here, we provide evidence indicating that S. aureus secretes small linear peptides into the environment via a novel processing and secretion pathway. The discovery of a specialized pathway for the production of small linear peptides and the identification of these peptides leads to several important questions regarding their role in S. aureus biology, most interestingly, their potential to act as signaling molecules. The observations in this study provide a foundation for further in-depth studies into the biological activity of small linear peptides in S. aureus.

Wael Salem, Benjamin Cellini, Mark A. Frye, and Jean-Michel Mongeau

Most animals shift gaze by a ‘fixate and saccade’ strategy, where the fixation phase stabilizes background motion. A logical prerequisite for robust detection and tracking of moving foreground objects, therefore, is to suppress the perception of background motion. In a virtual reality magnetic tether system enabling free yaw movement, Drosophila implemented a fixate and saccade strategy in the presence of a static panorama. When the spatial wavelength of a vertical grating was below the Nyquist wavelength of the compound eyes, flies drifted continuously­ and gaze could not be maintained at a single location. Because the drift occurs from a motionless stimulus—thus any perceived motion stimuli are generated by the fly itself—it is illusory, driven by perceptual aliasing. Notably, the drift speed was significantly faster than under a uniform panorama suggesting perceptual enhancement due to aliasing. Under the same visual conditions in a rigid tether paradigm, wing steering responses to the unresolvable static panorama were not distinguishable from a resolvable static pattern, suggesting visual aliasing is induced by ego motion. We hypothesized that obstructing the control of gaze fixation also disrupts detection and tracking of objects. Using the illusory motion stimulus, we show that magnetically tethered Drosophila track objects robustly in flight even when gaze is not fixated as flies continuously drift. Taken together, our study provides further support for parallel visual motion processing and reveals the critical influence of body motion on visuomotor processing. Motion illusions can reveal important shared principles of information processing across taxa.

The arrangement of functionally-related genes in operons is a fundamental element of how genetic information is organized in prokaryotes. This organization ensures coordinated gene expression by co-transcription. Often, however, alternative genetic responses to specific stress conditions demand the discoordination of operon expression. During cold temperature stress, accumulation of the gene encoding the sole Asp–Glu–Ala–Asp (DEAD)-box RNA helicase in Synechocystis sp. PCC 6803, crhR (slr0083), increases 15-fold. Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs. This cleavage event is required for and results in destabilization of the rimO transcript. Results from secondary structure modeling and analysis of RNase E cleavage of the rimO–crhR transcript in vitro suggested that CrhR plays a role in enhancing the rate of the processing in an auto-regulatory manner. Moreover, two putative small RNAs are generated from additional processing, degradation, or both of the rimO transcript. These results suggest a role for the bacterial RNA helicase CrhR in RNase E-dependent mRNA processing in Synechocystis and expand the known range of organisms possessing small RNAs derived from processing of mRNA transcripts.

Long before NLP became a hot field in AI, people devised rules and machines to manipulate language

A federal grand jury in Greensboro, N.C., returned an indictment today charging a poultry processor and a plant manager with multiple violations of the Clean Water Act for illegally discharging wastewater from its Raeford, N.C., based facility

The Department of Justice filed a civil antitrust lawsuit today challenging George’s Inc.’s acquisition of Tyson Foods’ Harrisonburg, Va., chicken processing complex.

Swift Beef Company, a subsidiary of JBS S.A, the world’s largest beef producer, has agreed to pay $1.3 million to the United States and state of Nebraska to settle alleged violations of the federal Clean Water Act and Nebraska state law at its Grand Island, Neb., beef processing plant.

Two men were arrested late last night and are charged by criminal complaint with terrorism and firearms related charges.

The Justice Department today filed a lawsuit against Mar-Jac Poultry Inc., a poultry processing plant in Gainesville, Ga., alleging that Mar-Jac requires all newly hired non-U.S. citizens to present documents issued by the Department of Homeland Security in order to secure their jobs, but the company does not require U.S. citizens to show any specific documentation.

Trident Seafoods Corp., one of the world’s largest seafood processors, has agreed to pay a $2.5 million civil penalty and invest millions in seafood processing waste controls to settle alleged violations of the Clean Water Act.

Walli Mujahidh, aka “Frederick Domingue, Jr.,” 32, pleaded guilty to conspiracy to murder officers and agents of the United States, conspiracy to use weapons of mass destruction and unlawful possession of a firearm.

The United States today obtained an agreed permanent injunction against a Chicago food processing firm, Triple A Services Inc., and three of its executives after filing a federal lawsuit alleging that Triple A’s ready-to-eat sandwiches and produce were not being prepared in compliance with federal regulations to protect food against contamination.

Columbus Manufacturing Inc., a wholly owned subsidiary of Columbus Foods LLC, has agreed to pay a penalty and make significant upgrades to settle Clean Air Act violations.

The U.S. District Court for the Eastern District of Washington has entered a consent decree against Dominguez Foods of Washington Inc. to resolve a food seizure action alleging violations of the Food, Drug and Cosmetic Act (FDCA), the Justice Department announced today.

A federal jury today found House of Raeford Farms Inc., the owner and operator of a poultry slaughtering and processing facility located in Raeford, North Carolina, guilty of 10 counts of knowing violations of the Clean Water Act.

A former executive of Lender Processing Services Inc. (LPS) – a publicly traded company based in Jacksonville, Fla. – pleaded guilty today, admitting her participation in a six-year scheme to prepare and file more than 1 million fraudulently signed and notarized mortgage-related documents with property recorders’ offices throughout the United States.

Lender Processing Services Inc. (LPS), a publicly traded mortgage servicing company based in Jacksonville, Fla., has agreed to pay $35 million in criminal penalties and forfeiture to address its participation in a six-year scheme to prepare and file more than 1 million fraudulently signed and notarized mortgage-related documents with property recorders’ offices throughout the United States.

A former executive of Lender Processing Services Inc. (LPS) – a publicly traded company based in Jacksonville, Fla. – was sentenced today to serve five years in prison for her participation in a six-year scheme to prepare and file more than 1 million fraudulently signed and notarized mortgage-related documents with property recorders’ offices throughout the United States.

According to court documents submitted today, BNP Paribas S.A. (BNPP), a global financial institution headquartered in Paris, agreed to enter a guilty plea to conspiring to violate the International Emergency Economic Powers Act (IEEPA) and the Trading with the Enemy Act (TWEA) by processing billions of dollars of transactions through the U.S. financial system on behalf of Sudanese, Iranian, and Cuban entities subject to U.S. economic sanctions

Instead of tossing it, fisheries should capture the protein and fat left in water and use it to feed humans and animals a second time around.

The report showcases some of the leading global businesses in Black Soldier Fly production.

Company: RM Infotech
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor in Hotel Management (B.H.M),....

Company: RM Infotech
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor in Hotel Management (B.H.M),....

Company: RM Infotech
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor in Hotel Management (B.H.M),....

Company: Nanu India Recruitment Co
Qualification: Bachelor Of Technology (B.Tech/B.E), Diploma
Experience: 0 to 2
Salary: 1.70 to 4.00
location: Delhi, Gurgaon / Gurugram
Ref: 24824706
Summary: We have Requirement for Production - Food Processing Engineer. Freshers also can apply.

Company: RM Infotech
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor in Hotel Management (B.H.M),....

Company: RM Infotech
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor in Hotel Management (B.H.M),....

Company: RM Infotech
Qualification: Bachelor of Arts (B.A), Bachelor of Architecture (B.Arch), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor in Hotel Management (B.H.M),....

Company: Confidential
Qualification: Bachelor of Arts (B.A), Bachelor of Business Administration (B.B.A), Bachelor Of Computer Application (B.C.A), Bachelor of Commerce (B.Com), Bachelor Of Technology (B.Tech/B.E), Bachelor of Education (B.Ed), Bachelor in Hotel Management (B.H.M), Bachelors of Law (B.L/L.L.B), Bachelor....

Imports of Typewriters & Word Processing Machines in Mexico increased to 35 USD THO in March from 2 USD THO in February of 2020. Imports of Typewriters & Word Processing Machines in Mexico averaged 23.94 USD THO from 2014 until 2020, reaching an all time high of 95 USD THO in May of 2016 and a record low of 0 USD THO in July of 2016. This page includes a chart with historical data for Mexico Imports of Typewriters & Word Processing Machines.

Imports - Specialized Mining & Oil Processing Eqp. (Census) in the United States increased to 83.37 USD Million in March from 68.77 USD Million in February of 2020. Imports - Specialized Mining & Oil Processing Eqp. in the United States averaged 44.60 USD Million from 1989 until 2020, reaching an all time high of 106.51 USD Million in March of 2007 and a record low of 11.50 USD Million in January of 1993. This page includes a chart with historical data for the United States Imports of Specialized Mining & Oil Processing Eq.

Fears of food shortages as coronavirus forces facilities to shut and US cases top 1m

The number of coronavirus cases linked to a Melbourne meat processing factory as grown to 62 after 13 new cases.

US banks earned $10 billion in two weeks processing the loans from the government scheme to protect small businesses from financial ruin during the coronavirus crisis, according to an NPR report.The rescue plan worth $349 billion offered businesses loans of up to $10 million to thousands of US companies and were guaranteed by the federal Small Business Administration.The banks charged a transaction fee of 5% on loans worth less than $350,000, while on loans worth between $2 million - $10 million, the cost was 1%.The banks defended the massive windfall of loan transaction fees, saying that processing the loans involved complicated vetting procedures.Treasury Department guidelines are less rigorous than for regular loans, and the taxpayer provides the funding, so there is little risk for the

The 'Fold@Home' technology involves users downloading an app for their PC hat runs in the background, letting it use any processing power they're not using.

The government has announced 100% FDI in marketing of food products produced and processed in India in the Budget

Amid the coronavirus pandemic, the retirement fund body Employees' Provident Fund Organisation (EPFO) has granted provision to allow withdrawal of funds from the fund balance to tackle liquidity crunch. Nonetheless, while the institution has received many such withdrawal request there has