Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affects circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDL were isolated from patients with severe obesity (n=53) before, 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDL were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222 and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222 and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222 and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase (PON1) activity were increased and intracellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with increase in cholesterol efflux capacity (r=0.326, P=0.027 and r=0.349, P=0.017 respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction, and may lead to the development of novel treatment strategies for obesity and related metabolic disorders.

This article has been withdrawn by the authors. This article did not comply with the editorial guidelines of MCP. Specifically, single peptide based protein identifications of 9-19% were included in the analysis and discussed in the results and conclusions. We wish to withdraw this article and resubmit a clarified, corrected manuscript for review.

With less than a week left until Angels pitchers and catchers report to Spring Training, it's time for the final Angels Inbox of the offseason.

NASA is inviting pubic participation in some virtual activities to watch the SpaceX space station resupply mission scheduled for a Monday night liftoff from NASA's Kennedy Space Center in Florida.

A bill prohibiting anti-Semitism in Florida's public schools and universities is going to Republican Gov. Ron DeSantis.

"Le Lyonnais" descended into the depths off the coast of Massachusetts after colliding with the "Adriatic," a sailing vessel that left the floundering steamship to fend for itself

Ten years after their formation, the members of The Brooks discuss how they’ve evolved and how they view their collaboration.

As a child, the future president acquired a marine animal's skull, which became the first specimen in his natural history collection

Max Topolnisky, a 100-year-old Second World War veteran, was out selling poppies for the Lockerby Legion in Sudbury, Ont., two days before he died, on Nov. 1, 2024.

3D ContentCentral Community Reaches 1 Million Users; Launch of MySolidWorks, a New Free Service that Aggregates Community Knowledge

Apple now has an official password manager, but importing your old passwords from other apps into Apple Passwords can be a bit of a pain.

The introduction of iOS 18 and macOS Seqouia has seen the old iCloud Keychain be rolled into the new Apple Passwords app, alongside a few other Apple password management features. If you already use a password manager outside of iCloud Keychain, you'll probably want to import your passwords if you intend on using Apple Passwords.

Do note that Apple's new Passwords app is really built for storing login passwords. It does not, as yet, store the items like credit card numbers, software serial numbers, or secure documents, that other password managers too.


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Balboa, Panama :: Logos Hope's volunteers make an uplifting visit to a shelter for migrant men, testing their eyesight and handing out reading glasses.

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For years, Gustavo, an architect from Central America, felt drawn to working in the Arabian Peninsula. Then, on a short-term trip, he saw what it could be like to use his profession overseas.

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Russian MPs on Tuesday passed in the final third reading controversial legislation banning "propaganda" of remaining childless, the latest measure targeting what Moscow depicts as Western liberal ideas.

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The Compass Grant is a brand new grant program from the Delaware Department of State that helps Delaware small businesses navigate selling their products or services around the world.   Compass Grant Informational Webinar Tuesday, December 12, 2023 at 10:00 AM   Are you seeking financial support to help your organization grow your sales overseas? […]

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Attorney General Kathy Jennings released Tuesday the following statement regarding news of the tragic passing of former Election Commissioner Elaine Manlove and her husband: It is especially painful to learn of Elaine and Wayne’s passing on Election Day — a day that she dedicated so much of her career to administering and defending. Elaine was […]

Legislation passed by the State Senate on Tuesday is one step away from ensuring Delawareans are protected from unfair business practices by a statute as protective as the law in nearly every other state. House Bill 91, sponsored by Rep. David Bentz, D-Newark, and Sen. Trey Paradee, D-Dover, amends Delaware’s Consumer Fraud Act to explicitly add unfair practices to the list of prohibited practices. […]

The State Senate unanimously passed legislation Wednesday that would formalize a responsible, independent, and collaborative process to allocate the significant funds the State has received, and will continue to receive, from opioid settlements. Senate Bill 166, sponsored by Sen. Stephanie Hansen and House Speaker Pete Schwartzkopf, and supported by Attorney General Kathy Jennings, would establish […]

The First State is one giant step closer to being a national leader on transparency.  I’m thrilled that more than a year’s worth of hard work and dedication now means that a statewide body camera program heads to the Governor’s desk. This will mean stronger community relationships, trust, and accountability. This legislation makes Delaware a […]

Today Delaware is ready to fix one of America’s weakest use-of-force laws, period. This issue has come up again and again throughout our state’s debate on police reform. The need for reform is overwhelming: not only the Department of Justice, but police reform advocates and the police themselves all recognize the need to better align […]

Attorney General Kathy Jennings today released the following statement regarding the Senate’s overwhelming passage of Senate Bill 2, legislation requiring training and a permit to purchase a handgun. The Senate’s vote sends the legislation to Gov. John Carney’s desk.  For five years, all of us — not only me, but the bill sponsors, the advocates, […]

Delaware fruit and vegetable growers who receive a passing score on their third-party food safety audits and agricultural water tests are eligible to receive cost-share to help cover the expense through the Delaware Department of Agriculture.

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It is most significant to note that while leading from the front, the Allahabad High Court in a most learned, laudable, landmark, logical and latest judgment titled Hazi Tahseen Khan vs Gandhi Mohan Saxena And Another in S.C.C. Revision No.: - 105 of 2024 that was pronounced as recently as on 07.11.

Delaware Office of Highway Safety Promotes National Child Passenger Safety Week from September 20 – 26, 2020 FOR IMMEDIATE RELEASE Media Contacts: Cynthia Cavett, Marketing Specialist & Public Information Officer Delaware Office of Highway Safety Cynthia.Cavett@delaware.gov 302-744-2743 DOVER, Del. (September 21, 2020) – Child Passenger Safety Week is a nationwide campaign that spreads critical awareness and […]

Media Contact: Veronica Marshall Traffic Safety Outreach Coordinator Veronica.Marshall@Delaware.Gov Car crashes are a leading cause of death for children – let’s change that OHS Promotes National Child Passenger Safety Week September 19 – 25, 2021 [DOVER, Del.] September 15, 2021 — Car crashes are a leading cause of death for children, according to the latest National […]

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DOVER, Del. – Governor John Carney on Thursday issued the following statement on the passage of Senate Bill 325 and Senate Bill 326: “This budget continues our investments in Delaware’s public schools, workforce, and environment, while doing so responsibly and sustainably. We are continuing to raise salaries for teachers, provide more resources for students who need […]