Hundreds congregated in downtown St. John's Monday morning to pay tribute to Newfoundland and Labrador's fallen soldiers, and honoured the dead for the first time at the newly refurbished National War Memorial and tomb of the Unknown Solider. 

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Pat White, an entrepreneur who launched EVAS Air and the Gander Flight Training, has died. He was 69 years old.

The 50th Christmas Craft Fair gathered over a hundred vendors and thousands of customers in Torbay over the weekend. One family business was inspired by the early years of the fair and a passion to keep traditional crafts in style.

CBC Newfoundland and Labrador would love to hear your suggestions of folks and organizations who are there when you need them — and all the other times, too.

People using the library in the Arts and Culture Centre in St. John's are being forced to use alternate entrances due to an unsafe staircase in front of the building.

A woman who says she was repeatedly denied emergency care last spring is blasting the Newfoundland and Labrador health-care system, saying she’s been left psychologically scarred after being told several times that her gangrenous appendix was simply anxiety or constipation.

Ashley Hickey, a parent of a kid with celiac disease, is concerned that Nova Scotia's new school program does not offer any 100 per cent guaranteed gluten-free options.

A researcher studying the rise of extremism in Atlantic Canada warns Ku Klux Klan Halloween costumes are just one example of an insidious effort by far-right groups to normalize hateful attitudes.

A candidate for the Nova Scotia NDP has apologized and is no longer running for the party in the provincial election after her comments on Israel and the Holocaust drew concern from the Jewish organizations. 

Independent Elizabeth Smith-McCrossin is back on the campaign trail in Cumberland North, but opponents say the riding would be better represented by an MLA from an official party. 

A Port Hawkesbury woman has long imagined a Remembrance Day with her great-uncle's war medals. It will happen this year for the first time thanks to some sleuthing and the kindness of her great-uncle's foster family.

Kate MacEachern is a Canadian veteran from Ballantynes Cove, N.S., but her service years are far from over. She has spent about two years living in Ukraine, delivering humanitarian aid to communities across the country.

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About a thousand people stood solemnly in the rain at a cenotaph in downtown Halifax on Monday morning to pay tribute to veterans on Remembrance Day.

As Nova Scotia politicians make their fall election promises, Julie Leggett is urging them to commit to helping people on income assistance stay cool during heat waves.

The only daily TV news package to focus on Nova Scotians and their stories

Building highways isn't the only solution to the growing problem of traffic congestion in and around the Halifax Regional Municipality, says the head of an organization that advocates for rail-based public transit.

The new mayor and council are planning big changes for the Cape Breton Regional Municipality, and one of those is at the top of the staff organizational chart now that chief administrative officer Marie Walsh is set to retire.

The Montreal English Theatre Awards ceremony is being held this evening at Le Gesù theatre. For the community, it's a time to celebrate their achievements and resilience coming out of the pandemic, together.

Dominic April fell in love with forging after his career in the military came to an end in 2015. Now operating a blacksmith studio near Quebec City, he hopes to help other veterans dealing with PTSD find creativity and purpose.

The Société de transport de Montréal (STM) has established a non-urgent text line for transit users to signal security issues in the Metro network.

Shilei Du, 36, was charged Monday morning with the premeditated murder of his partner, Guangmei Ye, whose body was found Sunday in a residence on Fontenelle Street in Candiac, Que.

Loved ones, friends, and people from across the city have all come together to support each other at Pastel Rita, the café Jocelyn Després helped so many call home.

The union representing dockworkers in Montreal says Canada's labour minister forcing its members back to work at ports in Montreal and Quebec City marks a "dark day for workers' rights."

Quebec provincial police have confirmed that the body found in a Montreal nature park on Oct. 30 was that of kidnapping victim and cryptocurrency influencer Kevin Mirshahi.

Amid rapid global advances and deployment of artificial intelligence technologies, the federal government has invested millions to combine the minds of three existing institutes into one that can keep an eye on potential dangers ahead.

Mosquito-borne disease is a major global public health issue. One path toward the development of evidence-based strategies to limit mosquito biting is the study of the mosquito nervous system—in particular, the sensory systems that drive biting behavior. The central nervous system of insects consists of the brain and the ventral nerve cord. Here, we describe a protocol for dissecting, immunofluorescent labeling, and imaging both of these structures in the mosquito. This protocol was optimized for Aedes aegypti and works well on Anopheles gambiae tissue. It has not been tested in other mosquito species, but we anticipate that it would work on a range of mosquitoes, and, if not, our protocol will provide a starting point from which to optimize. Notably, a limited number of antibodies cross-react with Ae. aegypti proteins. This protocol is intended for use with validated antibodies and can also be used to test new antibodies as they are generated. It has been successfully used to visualize protein tags, such as green fluorescent protein, that have been introduced into the mosquito to amplify or detect their presence.

Genetically encoded calcium indicators (GECIs) allow for the noninvasive evaluation of neuronal activity in vivo, and imaging GECIs in Drosophila has become commonplace for understanding neural functions and connectivity in this system. GECIs can also be used as read-outs for studying sleep in this model organism. Here, we describe a methodology for tracking the activity of neurons in the fly brain using a two-photon (2p) microscopy system. This method can be adapted to perform functional studies of neural activity in Drosophila under both spontaneous and evoked conditions, as well as during spontaneous or induced sleep. We first describe a tethering and surgical procedure that allows survival under the microscopy conditions required for long-term recordings. We then outline the steps and reagents required for optogenetic activation of sleep-promoting neurons while simultaneously recording neural activity from the fly brain. We also describe the procedure for recording from two different locations—namely, the top of the head (e.g., to record mushroom body calyx activity) or the back of the head (e.g., to record central complex activity). We also provide different strategies for recording from GECIs confined to the cell body versus the entire neuron. Finally, we describe the steps required for analyzing the multidimensional data that can be acquired. In all, this protocol shows how to perform calcium imaging experiments in tethered flies, with a focus on acquiring spontaneous and induced sleep data.

Sleep studies in Drosophila melanogaster rely mostly on behavioral read-outs to support molecular or circuit-level investigations in this model. Electrophysiology can provide an additional level of understanding in these studies to, for example, investigate changes in brain activity associated with sleep manipulations. In this protocol, we describe a procedure for performing multichannel local field potential (LFP) recordings in the fruit fly, with a flexible system that can be adapted to different experimental paradigms and situations. The approach uses electrodes containing multiple recording sites (16), allowing the acquisition of large amounts of neuronal activity data from a transect through the brain while flies are still able to sleep. The approach starts by tethering the fly, followed by positioning it on an air-supported ball. A multichannel silicon probe is then inserted laterally into the fly brain via one eye, allowing for recording of electrical signals from the retina through to the central brain. These recordings can be acquired under spontaneous conditions or in the presence of visual stimuli, and the minimal surgery promotes long-term recordings (e.g., overnight). Sleep and wake can be tracked using infrared cameras, which allow for the measurement of locomotive activity as well as microbehaviors such as proboscis extensions during sleep. The protocol has been optimized to promote subject survivability, which is an important factor when performing long-term (~16-h) recordings. The approach described here uses specific recording probes, data acquisition devices, and analysis tools. Although it is expected that some of these items might need to be adapted to the equipment available in different laboratories, the overall aim is to provide an overview on how to record electrical activity across the brain of behaving (and sleeping) flies using this kind of approach and technology.

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

After more than a century of searching, scientists have discovered a gene in snails that may control asymmetries inside many animals

When the National Transportation Safety Board office in Alaska first hears about a plane going down, protocol is to begin gathering information

A Two-Day Festival in the historic Arts & Industries Building brings community, artists and scholars together for a “Culture Lab”

On this episode of Generation Anthropocene, we dive into one of the most underappreciated elements on the periodic table

An unlikely friendship with Guy Burgess, the infamous British double-agent, brought unexpected joy to Stanley Weiss

The ancient burial structures, strikingly similar all across Europe and the mediterranean, have puzzled scientists and historians for centuries

The Northwest Territories Power Corporation expects that by the end of March it will have generated 72 per cent of power for the North Slave region using hydro, and 28 per cent from diesel. Last year, about half the region's power was from diesel because of low water levels.

More than 50 Norman Wells residents gathered at the Royal Canadian Legion on Thursday night to voice frustrations over high heating fuel prices.

Tammy Roberts, Home Base's executive director, said before these services launched, there was "no place for youth to go" other than the emergency room or to RCMP cells. 

Former Dawson City Mayor Bill Kendrick has filed his defence against the town's encroachment violation lawsuit.

With president-elect Donald Trump promising to drill in Alaska's Arctic National Wildlife Refuge, the Gwich'in are preparing to fight for the Porcupine caribou herd.

Wanita Johnson, who celebrates her birthday this weekend in Whitehorse, still has a sharp memory for how the Second World War helped shape her life.

Paraglider Ben Lewis remembers spinning around wildly as he got pulled up higher and higher into the violent storm. That was when he realized, about 6,700 metres up in the sky and still rising, that he was not likely to see his family again.

Yukon appeal court Justice Karen Horsman granted a temporary stay of the requirement on Friday, the latest development in an ongoing legal battle over the property between the territorial government, cabin-owner Len Tarka and tenant Eric DeLong. 

Stephanie Yuill spent five weeks visiting First World War sites across Europe on a bike she bought from the grocery store. 

As people across the country mourn the Honourable Justice Murray Sinclair, some in the Northwest Territories justice system are reflecting on his contributions to the country — one of those being Gladue principles. 

A Whitehorse homeowner has been charged in connection with an explosion last year that destroyed his home and killed a man in a neighbouring house. 

Hay River RCMP say they believe two weekend incidents, including one in which a person was shot, are linked to drugs and organized crime.