Posted by: laughing_tree



Expect evil ravens, hipsters performing pun-magic and John getting drunker than you’ve ever seen him before. Fun. -- Si Spurrier

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How many stupid, outrageous, maddening government failures can you document in just 500 words? Jim Doti and my former colleague Laurence Iannaccone should win a prize for this piece in the WSJ: …the U.S. is, by far, the world’s largest producer of alcohol. That distinction is a result of the Energy Policy Act of 2005, […]

The post World’s Largest Producer of Rubbing Alcohol Can’t Manufacturer Hand Sanitizer appeared first on Marginal REVOLUTION.

Welcome to the first episode of the Internet Musician Podcast! Show notes for Episode #1: Subscribe to the Internet Musician Podcast with iTunes: The first episode of The Internet Musician Podcast, hosted by indie artist and internet music marketing junkie Brian Hartzog (http://www.brianhartzog.net).  In this introductory episode, Brian introduces himself, his indie music credentials and […]

Focusing on urbanization as a key driver of environmental change in the 21st century, researchers at Princeton have created a framework to understand and compare cities’ food systems and their effects on climate change, water use and land use. The research will allow planners to estimate the impact of a city’s food system and evaluate policy actions.

President Trump on Friday called the viral video of Ahmaud Arbery’s killing “very, very disturbing,” but he said he’s confident that Georgia’s law enforcement authorities will handle the case properly despite a slow-moving investigation and conflict-of-interest questions surrounding the incident. “So I saw the tape, and it’s very, very disturbing," he said in a Fox New phone interview. “I looked at a picture of that young man. He was in a tuxedo... And I will say that that looks like a really good, young guy.”

The desperate search for two teens who vanished while tubing in Utah continued on Saturday, days after the pair were swept up in an intense storm.

Mamadou Bah, 19, an immigrant from Senegal who dreamt of playing pro basketball, died after he stumbled into Gentlemen’s Quarters Salon on Church Ave. near Ocean Ave. in Flatbush just before 10 p.m. Tuesday.

President Trump on Friday called the viral video of Ahmaud Arbery’s killing “very, very disturbing,” but he said he’s confident that Georgia’s law enforcement authorities will handle the case properly despite a slow-moving investigation and conflict-of-interest questions surrounding the incident. “So I saw the tape, and it’s very, very disturbing," he said in a Fox New phone interview. “I looked at a picture of that young man. He was in a tuxedo... And I will say that that looks like a really good, young guy.”

"Never in my wildest dreams did I think it would be my brother until I saw everything the next day and saw his name," said Rosemary Gomez, 38. “I was like, ‘No, it can’t be him, it can’t be him.' Then his (estranged) wife called me. She was in a state of shock."

President Trump on Friday called the viral video of Ahmaud Arbery’s killing “very, very disturbing,” but he said he’s confident that Georgia’s law enforcement authorities will handle the case properly despite a slow-moving investigation and conflict-of-interest questions surrounding the incident. “So I saw the tape, and it’s very, very disturbing," he said in a Fox New phone interview. “I looked at a picture of that young man. He was in a tuxedo... And I will say that that looks like a really good, young guy.”

The desperate search for two teens who vanished while tubing in Utah continued on Saturday, days after the pair were swept up in an intense storm.

The artist, musician and iconoclast — best known as part of the influential industrial band Throbbing Gristle — had been battling leukemia.

In 'Upload,' a new Amazon comedy from 'The Office' and 'Parks and Recreation' whiz Greg Daniels, Robbie Amell stars as a man in a (digital) afterlife.

At McKinstry, we're proud to be a collective group of innovators and problem solvers, builders and engineers, mentors and students. We strive to create an engaging work environment that empowers you to do the work you are passionate about. We believe deeply that energy and facility services, engi

The suspect is a 21-year-old Noblesville man who is facing three felony charges.

Police charge David Egan with the murder of Danny Pearce in Greenwich, south-east London.

US police say Bing Liu was shot dead by his lover, but conspiracy theorists have other ideas.

“Star Wars: The Rise of Skywalker” is now available to buy, but we suggest you skip it and go in this order instead.

Hear about climbing Mount Kilimanjaro as the Amateur Traveler talks to Kylie Chenn from Acanela Expeditions about what you should know about climbing the tallest mountain in Africa. 

Sen. Ted Cruz was apparently about to get into mullet territory. He hadn’t had a haircut in three months. Things were looking grim for the Texas senator. Thankfully, Shelley Luther is able to do something about that. Luther is both famous and free after a viral clip of her standing up to a judge who…

The post Ted Cruz Stops by Shelly Luther’s Salon for a Trim, ‘True Authenticity’ Leaves Her Sobbing appeared first on The Western Journal.

'Hollywood' actor Samara Weaving lets us in on all of the movies and television she's been watching during quarantine.   Read more...

The following article, Univ of Pittsburg Researcher, China Native, Dr. Bing Liu, “On the verge” of COVID19 Breakthrough Is Murdered…Alleged Gunman, Hao Gu, Kills Himself, was first published on 100PercentFedUp.com.

A 37-year-old China native and "outstanding researcher" at the University of Pittsburgh...

Continue reading: Univ of Pittsburg Researcher, China Native, Dr. Bing Liu, “On the verge” of COVID19 Breakthrough Is Murdered…Alleged Gunman, Hao Gu, Kills Himself ...

Imagine trying to describe the circulation and temperatures across the vast expanse of our oceans. Good models of our oceans not only benefit fishermen on our coasts but farmers inland as well. Until recently, there were neither adequate tools nor enough data to construct models. Now with new data and new mathematics, short-range climate forecasting for example, of an upcoming El Nino is possible.There is still much work to be done in long-term climate forecasting, however, and we only barely understand the oceans. Existing equations describe ocean dynamics, but solutions to the equations are currently out of reach. No computer can accommodate the data required to approximate a good solution to these equations. Researchers therefore make simplifying assumptions in order to solve the equations. New data are used to test the accuracy of models derived from these assumptions. This research is essential because we cannot understand our climate until we understand the oceans. For More Information: What.s Happening in the Mathematical Sciences, Vol 1, Barry Cipra.

Below, Mac Hyman, Tulane University, talks about types of mathematical models--their strengths and weaknesses--the data that we currently have and what we really need, and what models can tell us about a possible second wave.

At the beginning of the video, he thanks the mathematics community for its work, and near the end says, "Our mathematical community is really playing a central role in helping to predict the spread, and help mitigate this epidemic, and prioritize our efforts. …Do not underestimate the power that mathematics can have in helping to mitigate this epidemic—-we have a role to play."

See the full set of videos on modeling COVID-19 and see media coverage of mathematics' role in modeling the pandemic.

The police are probing the shooting of two homeless persons along Church Street in downtown Kingston. The incident, which happened about 10:10 Thursday morning, drew a large gathering. The Gleaner understands that both injured persons were rushed...

Non-medical interventions are increasingly being proposed to address wider determinants of health and to help patients improve health behaviours and better manage their conditions - this is known as social prescribing. In England, the NHS Long Term Plan states that nearly one million people will qualify for referral to social prescribing schemes...

We’re back for the December Talk Evidence, and this month we’re being very digital Firstly,(1.20) Helen tells us about arthritic fingers - should we be using prednisolone for treatment when people have painful osteoarthritis of the hand Then (13.30) Carl gets us all to check our fingers for clubbing, and we find out how useful it is as a test...

This Transatlantic Council on Migration Statement assesses the continuum of policies needed to disrupt illegal migration-related activities and addresses the conditions that make them possible. It examines the role of migration "bad actors"—human traffickers and unscrupulous employers, among them—who operate and profit in this environment, and considers how governments can deploy resources to discourage their actions.

Here’s my Q&A column from the WSJ this week — and if you have any questions for me, you can tweet them to @danariely with the hashtag #askariely, post a comment on my Ask Ariely Facebook page, or email them to AskAriely@wsj.com. ___________________________________________________ Dear Dan, When I host friends for dinner,...

Coronavirus is spreading rapidly in pre- and post-trial correctional facilities across the United States, and the challenges of social distancing for students in regular districts are all massively compounded for students behind bars.

Oral history -- Methodology.

Berlin : Heuser, 1889.

Berlin : Fischer, 1891.

Berlin : Fischer, 1893.

Berlin : Hirschwald, 1896.

Leipzig : P. Hobbing, 1890.

London : Kegan Paul, Trench, Trubner, 1890.

Liverpool (82, Duke Street) ; And in London (13 St. James's Place, Hampstead Road) : Published ... by the artist, April 10th. 1854.

Brian Zingg
Apr 15, 2020; 40:3250-3267
Systems/Circuits

Revealing the organization and function of neural circuits is greatly facilitated by viral tools that spread transsynaptically. Adeno-associated virus (AAV) exhibits anterograde transneuronal transport, however, the synaptic specificity of this spread and its broad application within a diverse set of circuits remains to be explored. Here, using anatomic, functional, and molecular approaches, we provide evidence for the preferential transport of AAV1 to postsynaptically connected neurons and reveal its spread is strongly dependent on synaptic transmitter release. In addition to glutamatergic pathways, AAV1 also spreads through GABAergic synapses to both excitatory and inhibitory cell types. We observed little or no transport, however, through neuromodulatory projections (e.g., serotonergic, cholinergic, and noradrenergic). In addition, we found that AAV1 can be transported through long-distance descending projections from various brain regions to effectively transduce spinal cord neurons. Combined with newly designed intersectional and sparse labeling strategies, AAV1 can be applied within a wide variety of pathways to categorize neurons according to their input sources, morphology, and molecular identities. These properties make AAV1 a promising anterograde transsynaptic tool for establishing a comprehensive cell-atlas of the brain, although its capacity for retrograde transport currently limits its use to unidirectional circuits.

SIGNIFICANCE STATEMENT The discovery of anterograde transneuronal spread of AAV1 generates great promise for its application as a unique tool for manipulating input-defined cell populations and mapping their outputs. However, several outstanding questions remain for anterograde transsynaptic approaches in the field: (1) whether AAV1 spreads exclusively or specifically to synaptically connected neurons, and (2) how broad its application could be in various types of neural circuits in the brain. This study provides several lines of evidence in terms of anatomy, functional innervation, and underlying mechanisms, to strongly support that AAV1 anterograde transneuronal spread is highly synapse specific. In addition, several potentially important applications of transsynaptic AAV1 in probing neural circuits are described.

For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen. The blots developed with chromogenic substrates can also be stripped of antibodies and reprobed, but the bands detected in the first round of immunoblotting will remain unaffected. Stripping and reprobing of the membrane are particularly useful when the amount of sample is limited or when it is important to accurately compare the signal between two different protein antigens in the same sample. Examples of such experiments include determining the levels of a protein antigen in a series of samples relative to the loading control and comparison of the phosphorylated form to the total levels of the protein in the sample.

Bing Liu (date: 5/9/2020 - Rank: 9)

On 22 August, 150 people climbed in the inaugural OM Boonah Freedom Climb to raise awareness and funding for the Jogini girls of India.

A woman found not criminally responsible for fatally stabbing a stranger in the heart at the makeup counter of a Toronto drugstore five years ago could ultimately be allowed to live in the community if the mental health facility where she is staying decides she can, the Ontario Review Board says.

A man in Kamloops, B.C., has died and two others are in hospital with life-threatening injuries after a stabbing at a party at an apartment complex Saturday night.