Incorporation of ribonucleotides into DNA can severely diminish genome integrity. However, how ribonucleotides instigate DNA damage is poorly understood. In DNA, they can promote replication stress and genomic instability and have been implicated in several diseases. We report here the impact of the ribonucleotide rATP and of its naturally occurring damaged analog 1,N6-ethenoadenosine (1,N6-ϵrA) on translesion synthesis (TLS), mediated by human DNA polymerase η (hpol η), and on RNase H2–mediated incision. Mass spectral analysis revealed that 1,N6-ϵrA in DNA generates extensive frameshifts during TLS, which can lead to genomic instability. Moreover, steady-state kinetic analysis of the TLS process indicated that deoxypurines (i.e. dATP and dGTP) are inserted predominantly opposite 1,N6-ϵrA. We also show that hpol η acts as a reverse transcriptase in the presence of damaged ribonucleotide 1,N6-ϵrA but has poor RNA primer extension activities. Steady-state kinetic analysis of reverse transcription and RNA primer extension showed that hpol η favors the addition of dATP and dGTP opposite 1,N6-ϵrA. We also found that RNase H2 recognizes 1,N6-ϵrA but has limited incision activity across from this lesion, which can lead to the persistence of this detrimental DNA adduct. We conclude that the damaged and unrepaired ribonucleotide 1,N6-ϵrA in DNA exhibits mutagenic potential and can also alter the reading frame in an mRNA transcript because 1,N6-ϵrA is incompletely incised by RNase H2.

A discussion marking the 25th anniversary of the 1990 Immigration Act, where experts examinine the history of the legislation, how it was accomplished politically, and the stakeholders and issues that were critical to its passage.

With issues of immigration policy very much at the forefront of the 2016 elections, this annual conference offers policy and legal analysis on key immigration topics facing the country and the incoming administration and Congress in 2017.

The 13th annual Immigration Law & Policy Conference offered policy and legal analysis on key immigration topics, including: the election and the future of immigration policy; refugee resettlement in the United States; immigration detention and enforcement; and the impacts of the Supreme Court opinion in the important U.S. vs Texas case

A discussion featuring data on immigration trends and the agricultural workforce, and some of the adjustments that farm employers are making, including increased mechanization, improved wages and benefits, and the increased use of the H-2A program. University of California-Davis’s Phil Martin, along with researchers from the the U.S. Department of Agriculture, U.S. Department of Labor, present their findings on the foreign agricultural workforce in the United States, which is followed by comments from the President of Farmworker Justice on some of the policy implications.

Even as the 1.8 million number swirls in the discussion of how many DREAMers would be placed on a path to citizenship, proposals debated in the Senate in February 2018 would have resulted in the legalization of smaller numbers, as this commentary explains. It offers estimates of potential beneficiaries of several Senate proposals, including one backed by the White House, and analysis of key criteria.

Award winners were honored at the second annual awards ceremony in Washington, DC, on May 18, 2010.

Winners of the 2nd annual E Pluribus Unum Prizes awarded to exceptional immigrant integration initiatives by MPI's National Center on Immigrant Integration Policy are the Illinois New Americans Integration Initiative; the Latino Community Credit Union in Durham, NC; Tacoma Community House in Tacoma, WA; and Upwardly Global in San Francisco, CA. McDonald's Corp. of Oak Brook, IL, received the sole honorable mention. The Prizes seek to reward exemplary efforts that uphold and update the ideal of "out of many, one" and inspire others to take on the important work of helping immigrants and their children join the mainstream of U.S. society as well as build stronger ties between immigrants and the native-born. Award winners were honored at the second annual awards ceremony in Washington, DC, on May 18, 2010. Watch highlights of the ceremony here.

The winners of the Migration Policy Institute's 2012 E Pluribus Unum Prizes, honoring exceptional immigrant integration initiatives in the United States, discussed their work during a plenary luncheon on September 24, 2012 at the National Immigrant Integration Conference held in Baltimore, MD.

The winners of the Migration Policy Institute's 2012 E Pluribus Unum Prizes--ACCESS (the Arab Community Center for Economic and Social Services), Building Skills Partnership, Californians Together, and Citi Community Development--discussed their immigrant integration initiatives at the National Immigrant Integration Conference.

This awards ceremony honored the 2013 winners of the E Pluribus Unum Prizes, which provides $50,000 prizes to exceptional U.S. immigrant integration initiatives. The awardees took part in a panel discussion with White House and state officials, followed by remarks from Congressman Luis Gutierrez and U.S. Senator Amy Klobuchar.

In a year when immigration has been prominent in the headlines, the 14th annual Immigration Law and Policy Conference provides timely policy and legal analysis, with expert discussion covering the shifting immigration policy priorities under a new administration, including changes in immigration enforcement, border enforcement, refugee resettlement, the treatment of unaccompanied minors and their families, Temporary Protected Status, and DACA, along with the responses from the judicial and congressional branches, various stakeholders, and state and local governments. 

At a time of intense and fast-moving action on immigration, 2018's Immigration Law and Policy Conference offered an excellent opportunity to go beyond the headlines with thoughtful analysis from leading experts.

At the 15th annual Immigration Law and Policy Conference, held in October 2018, leading experts and government officials discussed the role that immigration is playing in the mid-term elections; how the courts are handling key immigration questions; and recent changes in the U.S. asylum system, border and interior enforcement, and regulations surrounding legal immigration.   

During his first 100 days in office, President Trump has taken a sweeping set of actions on immigration, ranging from imposing a travel ban to cutting refugee admissions, "extreme" vetting, and fortifying immigration enforcement at the border and in the U.S. interior. This fact sheet examines the major immigration actions taken to date, legal challenges, and related policy and personnel developments.

The 10th annual Immigration Law and Policy Conference featured keynotes by U.S. Senator John McCain (R-AZ) and former Mississippi Governor Haley Barbour, and panel discussions on the prospects for legislative action, policy options, and political mobilization; lessons learned from past immigration legislation and programs; and an assessment of current conditions at the U.S. border and considerations for future policy. 

As the 100-day milestone for the Trump administration approaches, MPI's Doris Meissner and Muzaffar Chishti are joined by the former Director of U.S. Immigration and Customs Enforcement, Julie Myers Wood, and former DHS Assistant Secretary for Policy and Planning C. Stewart Verdery for a discussion of the administration's track record to date on immigration, the policies articulated in its executive orders, legal challenges, reactions by publics and policymakers, and the possible long-term effects of these policies.

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Una llamada en español que analiza las dinámicas cambiantes y las cuestiones políticas relacionadas con la migración a través de México a los Estados Unidos.

Durante este seminario, ponentes presentaron hallazgos de un reciente informe que utiliza datos de agencias gubernamentales mexicanas, entrevistas con funcionarios clave y relatos de la sociedad civil para examinar el marco legal para la protección de menores no acompañados y su aplicación, al igual que las brechas entre este marco y su aplicación durante los procesos de detención, interrogación y alojamiento. El presidente de MPI, Andrew Selee, también expuso cómo el cambio en la dinámica política en Estados Unidos puede afectar las cuestiones migratorias con México, así como los efectos en la relación bilateral en medio de tensiones sobre el muro fronterizo, la renegociación del acuerdo del TLCAN y una cifra significativa de repatriaciones de migrantes mexicanos.

OBJECTIVE

To describe sociodemographic and clinical characteristics of youth and young adults with type 1 diabetes who endorsed suicidal ideations as part of routine depression screening and the results of their suicide risk assessments.

OBJECTIVE

To assess the impact of a telemedicine visit using the platform Diabetic compared with a face-to-face visit on clinical outcomes, patients’ health-related quality of life (HRQoL), and physicians’ satisfaction in patients with type 1 diabetes.

OBJECTIVE

To improve outcomes of patients with adult type 2 diabetes by decreasing HbA_1c undertesting, reducing the proportion of patients with poor glycemic control, and lowering mean HbA_1c levels using a quality improvement (QI) program.

OBJECTIVE

Reparixin is an inhibitor of CXCR1/2 chemokine receptor shown to be an effective anti-inflammatory adjuvant in a pilot clinical trial in allotransplant recipients.

By Michael Pershan, St. Ann’s School I. What do primary/secondary math educators think of the teaching that happens in colleges? And — the other way around — what do mathematics professors think of primary and secondary math teaching? I’m nearing … Continue reading →

The German Bundesliga released its full soccer schedule Thursday after German officials announced that the league is allowed to resume games after they were suspended due to the coronavirus pandemic.

UFC 249 will be sandwiched between 14 live baseball broadcasts out of South Korea and Taiwan this weekend for American sports fans to watch as they wait for major sports leagues to return.

Joseph Tran
Oct 1, 2012; 30:173-178
Practical Pointers

Heidi L. Gassner
Jul 1, 2003; 21:
Case Studies

William H. Herman
Apr 1, 2001; 19:
Case Studies

Joy Dugan
Oct 1, 2017; 35:232-238
Practical Pointers

Vanessa J. Briscoe
Jul 1, 2006; 24:115-121
Feature Articles

Joseph Largay
Jan 1, 2012; 30:25-26
Case Studies

American Diabetes Association
Jan 1, 2018; 36:14-37
Position Statements

Jan 1, 2006; 24:9-9
Patient Information

American Diabetes Association
Jan 1, 2019; 37:11-34
Position Statements

OBJECTIVE

This study investigates two-phase growth patterns in early life and their association with development of islet autoimmunity (IA) and type 1 diabetes (T1D).

OBJECTIVE

In children with type 1 diabetes (T1D), severe hypoglycemia (SH) is associated with poorer cognition, but the association of SH with cognitive function in late life is unknown. Given the increasing life expectancy in people with T1D, understanding the role of SH in brain health is crucial.

OBJECTIVE

To assess the contemporaneous prevalence of diabetic peripheral neuropathy (DPN) in people with type 1 diabetes (T1D) in Scotland and study its cross-sectional association with risk factors and other diabetic complications.

A California 14-year-old who graduated high school at the age of 13 said she has now been accepted into the music programs at eight California universities.

A Virginia man waiting to cash in his $1,000 winning lottery ticket ended up winning an additional $177,777 before collecting his prize.

The 16th annual conference features thoughtful policy and legal analysis and discussion of the most important immigration topics from leading government officials, attorneys, researchers, advocates, and others.

Former Auburn defensive tackle Derrick Brown agreed to his rookie contract with the Carolina Panthers on Friday.

A "Shoeless" Joe Jackson baseball card from 1910 sold at auction for $492,000 this week.

The first COVID-19 test using saliva samples that patients collect at home has been approved by the U.S. Food and Drug Administration.