The actress becomes the first wheelchair user ever to take home the coveted prize at the 73rd annual award show

‘Our goal is to provide full flexibility, 24/7,’ one resident said. ‘If you want to cut the lawn at 4 a.m., then you do it.’

It's not clear how the juvenile male ended up so far north, but experts suggest he was motivated by his appetite

On this day in 1912, a team found the remains of Robert Falcon Scott and the crew of the "Terra Nova" expedition. A would-be rescuer said he was forever haunted by the "horrible nightmare"

A new study has shattered historians' long-held assumptions about some of the people who died in Mount Vesuvius' eruption in 79 C.E.

During a release of captive-bred snails in September, researchers discovered wild-born individuals from the Partula tohiveana species—which had been considered extinct in the wild—marking a huge milestone in a global effort to save them

Sławomir and Szymon Milewski were searching for a Roman road when they stumbled upon the cache of coins, which is worth more than $120,000

The 1951 artwork, "La Grande Dame (The Cat Woman)," stands over six feet tall and features paintings of "hybrid creatures and lush dreamscapes"

The Cuban-American actor and producer has stars on Hollywood’s Walk of Fame, but this new honor pays tribute to his start as a musician in South Florida

From Setsubun in Japan to Fèt Gede in Haiti, these festivals relish in the macabre

These images from the Smithsonian Magazine Photo Contest capture quintessentially Cuban scenes

Eight years into living in Longyearbyen, on the Norwegian archipelago of Svalbard, I embrace the seasonal plunge into total darkness

In the late 19th century, city officials turned the final resting place for 10,000 souls into what's now Greenwich Village’s James J. Walker Park

Pedestrians in Montreal, Grand Rapids and other locations can time-travel thanks to installations that map historical scenes directly onto the cityscapes

You asked, we answered

Get acquainted with the Big Apple’s major sights by browsing through these remarkable travel photos submitted to our photo contest

"Fascinating, Captain"

In one reef, three million sea cucumbers released 64,000 metric tons of nutrient-packed poo back into the ecosystem

As of last Wednesday, at least 3,500 sea turtles have been rescued from freezing waters in the midst record-breaking winter storm

In a twist befitting its pages, the satirical, anti-establishment publication that delivered laughs and hijinks to generations of young readers gets the respect it always deserved with a new museum exhibition

A new book charts the history of pet cemeteries and honors the universal experience of grieving an animal companion

Renaissance paintings, medieval archives, cloistered orchards—how one Italian scientist is uncovering secrets that could help combat a growing agricultural crisis

It fell to Belle da Costa Greene, a Black woman whose racial identity was kept secret for decades, to catalog J.P. Morgan's immense collection of books and art

It’s easy to write bugs off as pests, but consider the ways in which they have positively impacted our lives

In the international competition, people with physical disabilities put state-of-the-art devices to the test as they race to complete the tasks of everyday life

Manufacturing a Bentley in the traditional way is a time-consuming nod to their brand heritage. Each hand-crafted wheel can take 4.5 hours to finish

Newfoundland filmmaker Justin Simms is releasing his latest film called Sons. It was prompted by the birth of his son and left him wondering how traditional masculine behaviour is learned.

Police have reported that a man was fatally electrocuted in Torbay on Saturday evening. Two others are being treated for injuries.

Large crowds came together at city hall in Corner Brook to lay wreaths and honour veterans on Monday. The ceremony portion was completed with a playing of the Last Post.

Hundreds congregated in downtown St. John's Monday morning to pay tribute to Newfoundland and Labrador's fallen soldiers, and honoured the dead for the first time at the newly refurbished National War Memorial and tomb of the Unknown Solider. 

Nurses' union president Yvette Coffey says more must be done to ensure young nurses stay in the province. Newfoundland and Labrador Health Services says work is underway to match students with job openings.

A woman who says she was repeatedly denied emergency care last spring is blasting the Newfoundland and Labrador health-care system, saying she’s been left psychologically scarred after being told several times that her gangrenous appendix was simply anxiety or constipation.

A candidate for the Nova Scotia NDP has apologized and is no longer running for the party in the provincial election after her comments on Israel and the Holocaust drew concern from the Jewish organizations. 

The L'nu Sipuk Kina'muokuom school observed Indigenous Veterans Day with an event on Friday. Students, faculty and staff got together to honour Mi’kmaw military veterans and RCMP members.

Independent Elizabeth Smith-McCrossin is back on the campaign trail in Cumberland North, but opponents say the riding would be better represented by an MLA from an official party. 

A Port Hawkesbury woman has long imagined a Remembrance Day with her great-uncle's war medals. It will happen this year for the first time thanks to some sleuthing and the kindness of her great-uncle's foster family.

Building highways isn't the only solution to the growing problem of traffic congestion in and around the Halifax Regional Municipality, says the head of an organization that advocates for rail-based public transit.

The new mayor and council are planning big changes for the Cape Breton Regional Municipality, and one of those is at the top of the staff organizational chart now that chief administrative officer Marie Walsh is set to retire.

Elections Nova Scotia is investigating PC candidate Susan Corkum-Greek's campaign to determine whether it broke the law by handing out gift cards on Saturday outside a Tim Hortons in Lunenburg, N.S. 

Dominic April fell in love with forging after his career in the military came to an end in 2015. Now operating a blacksmith studio near Quebec City, he hopes to help other veterans dealing with PTSD find creativity and purpose.

Shilei Du, 36, was charged Monday morning with the premeditated murder of his partner, Guangmei Ye, whose body was found Sunday in a residence on Fontenelle Street in Candiac, Que.

Two Falls (Nishu Takuatshina) was developed in Montreal by a local studio that collaborated closely with Indigenous designers and consultants.

The City of Montreal wants to sell more land to community organizations, housing co-ops and other groups to build off-market housing. 

Ten years after their formation, the members of The Brooks discuss how they’ve evolved and how they view their collaboration.

The union representing dockworkers in Montreal says Canada's labour minister forcing its members back to work at ports in Montreal and Quebec City marks a "dark day for workers' rights."

Mosquito-borne disease is a major global public health issue. One path toward the development of evidence-based strategies to limit mosquito biting is the study of the mosquito nervous system—in particular, the sensory systems that drive biting behavior. The central nervous system of insects consists of the brain and the ventral nerve cord. Here, we describe a protocol for dissecting, immunofluorescent labeling, and imaging both of these structures in the mosquito. This protocol was optimized for Aedes aegypti and works well on Anopheles gambiae tissue. It has not been tested in other mosquito species, but we anticipate that it would work on a range of mosquitoes, and, if not, our protocol will provide a starting point from which to optimize. Notably, a limited number of antibodies cross-react with Ae. aegypti proteins. This protocol is intended for use with validated antibodies and can also be used to test new antibodies as they are generated. It has been successfully used to visualize protein tags, such as green fluorescent protein, that have been introduced into the mosquito to amplify or detect their presence.

Laboratory study of field-collected mosquitoes can allow researchers to better understand the ways variation within and among mosquito populations shapes burdens of mosquito-borne disease. The Anopheles gambiae complex comprises the most important vectors of malaria, but it can be challenging to keep in the laboratory. For some species of mosquitoes, especially An. gambiae, it is very difficult to bring viable eggs into the laboratory. Instead, it is preferable to collect larvae or pupae and then transport them as carefully as possible back to the laboratory. This simple protocol allows a researcher to start new laboratory colonies from larvae or pupae collected from natural breeding sites or proceed directly to their planned experiments. The use of natural breeding sites provides additional reassurance that the resulting colonies are representative of natural populations.

Laboratory study of natural populations of mosquitoes can play a key role in determining the underlying causes of variation in burdens of mosquito-borne disease. Aedes aegypti is the main vector of the viruses that cause dengue, chikungunya, Zika, and yellow fever, making it a high priority for laboratory study. Ae. aegypti eggs provide an ideal starting point for new laboratory colonies. Eggs can be collected using ovicups, which are small plastic cups lined with seed-germination paper and partially filled with leaf-infused H₂O. Once collected, dry eggs will remain viable for months and can be safely transported long distances back to the laboratory as long as they are properly stored. This protocol provides step-by-step instructions for preparing for collecting, storing, and hatching Ae. aegypti eggs and has successfully yielded laboratory colonies from locations across both the native and invasive range of this species.

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.