Junkyards are one of those forgotten places we in the heating business must visit on occasion.

The freshly designed and manufactured stock of the immensely popular Nidai Kitetsu will be available starting the second week of December.

1915: March 25: Massacre at Urumiah: 200 Assyrians Burned in Church - Orthodox Bishop Hanged - Refugees Executed

Despite its major importance in human health, the metabolic potential of the human gut microbiota is still poorly understood. We have recently shown that biosynthesis of Ruminococcin C (RumC), a novel ribosomally synthesized and posttranslationally modified peptide (RiPP) produced by the commensal bacterium Ruminococcus gnavus, requires two radical SAM enzymes (RumMC1 and RumMC2) catalyzing the formation of four Cα-thioether bridges. These bridges, which are essential for RumC's antibiotic properties against human pathogens such as Clostridium perfringens, define two hairpin domains giving this sactipeptide (sulfur-to-α-carbon thioether–containing peptide) an unusual architecture among natural products. We report here the biochemical and spectroscopic characterizations of RumMC2. EPR spectroscopy and mutagenesis data support that RumMC2 is a member of the large family of SPASM domain radical SAM enzymes characterized by the presence of three [4Fe-4S] clusters. We also demonstrate that this enzyme initiates its reaction by Cα H-atom abstraction and is able to catalyze the formation of nonnatural thioether bonds in engineered peptide substrates. Unexpectedly, our data support the formation of a ketoimine rather than an α,β-dehydro-amino acid intermediate during Cα-thioether bridge LC–MS/MS fragmentation. Finally, we explored the roles of the leader peptide and of the RiPP precursor peptide recognition element, present in myriad RiPP-modifying enzymes. Collectively, our data support a more complex role for the peptide recognition element and the core peptide for the installation of posttranslational modifications in RiPPs than previously anticipated and suggest a possible reaction intermediate for thioether bond formation.

Join the Australia Pavilion at Perumin 2023. Perumin has been central to successful market entry and expansion strategies for many Australian METS firms. Attracting senior executives, mine superintendents, procurement managers, technical staff and potential partners, PERUMIN is seen as the gathering of the Peruvian mining industry.

Berkeley, CA : University of California Press, [2023]

Dr. Frumin has been endorsed by Marquis Who's Who as a leader in the medical industry

Create faster and better routines with Serumize's new line of precision skincare products that target your unique skin concerns whilst challenging us to approach beauty from a functionality standpoint.

Theme: “pictures taken at home” Prizes: Gold Prize: Marumi soft filter + Marumi Cross Filter Set (DHG Portrait Soft, Retro Soft, SF, SFII, Foggy Soft, [...]

The post Marumi Photo Contest “Pictures taken at home” appeared first on Photocompete.

Originally broadcast in September 2007, this is one of the most requested episodes we’ve ever produced. Poet Coleman Barks and the ‘modern-day mystic’ Andrew Harvey explore all the ways Rumi, a 13th-century Persian poet, speaks to 21st-century hearts and minds.

As I’ve mentioned on social media, I only have two works eligible for awards nomination from 2017: The Stone Sky, and my Uncanny short story Henosis. Last year was tough, so I didn’t get much writing done. I’m sure a lot of you can relate. But since people have asked for my thoughts on this… […]

Beyoncé and Jay-Z enjoyed some sweet family time with a beach picnic on Sunday. The couple were spotted with their twins, Sir and Rumi, one, while lounging around in The Hamptons.

Online Resource

Hattori, Harumi, author

Rotch Library - HT395.N5 O34 2019