A microphone holder for mounting a microphone on a drum includes a vertical plate having an inner side surface which is adapted to face the drum, and a microphone connecting device, a clip member, and a positioning plate connected fixedly to the vertical plate. The microphone connecting device is adapted to hold the microphone thereon. The clip member clamps a vertical bolt that interconnects a drum body and a drum skin frame. The positioning plate is disposed above the clip member, and has a horizontal top plate section with a bottom surface that is adapted to abut against an upper end of the vertical bolt, and two vertical side plate sections which extend respectively and integrally from two opposite sides of the top plate section, and which are adapted to flank a projection on the drum skin frame through which the vertical bolt extends so as to prevent rotation of the clip member on the vertical bolt.