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OSCE supports Ukrainian legal education practitioners in developing human rights-oriented approaches to their work

Twenty-five lecturers from law faculties at fifteen universities from around Ukraine, during a roundtable discussion supported by the OSCE Project Co-ordinator on 13 July 2016 in Kyiv, developed recommendations on teaching human rights within Master of Law university programmes.

The discussions focused on teaching the European Convention on Human Rights and the case-law of the European Court of Human Rights (ECtHR), with international experts from the Max Planck Institute Luxembourg for International, European and Regulatory Procedural Law, the Union University and the ECtHR highlighting ways of ensuring a human rights-based approach to legal education.

Participants exchanged syllabi, research materials and teaching methodologies and agreed to set up a platform to further develop the human rights law curricula. This community of practice will function on a permanent basis with further support from the OSCE Project Co-ordinator and with the participation of leading Ukrainian law schools.

The roundtable discussion was held as part of the Second Annual Inter-University Human Rights School on 11-15 July 2016, which was organized by the OSCE Project Co-ordinator in Ukraine in co-operation with the Lviv and Kyiv Universities.

The School was held as part of the OSCE Project Co-ordinator’s project on safeguarding Human Rights through the courts. The project is being implemented with the financial support of the Government of Canada, and follows on from the First Annual Inter-University School dedicated to the Right to Life held in 2015.

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OSCE supports workshop on risks-oriented approach in combating money laundering and financing of terrorism in Kyrgyzstan

ISSYK-KUL, Kyrgyzstan, 8 July 2016 – A five-day training course supported by the OSCE Centre in Bishkek on a risk-oriented approach in supervising activities related to anti-money laundering and combating the financing of terrorism concluded today in Bosteri village, in the Issyk-Kul region.

The aim of the workshop, which was organized by the State Financial Intelligence Service under the Government of the Kyrgyz Republic (SFIS) in partnership with the International Monetary Fund (IMF), was to update the relevant State agencies and financial institutions on the practical aspects of a risks-oriented approach in their work. Some 46 officials from law enforcement agencies, supervisory agencies and financial institutions took part.

“A risks-oriented approach is the process which allows the countries to identify, assess and understand risks to effectively combat money laundering and financing of terrorism,” said Nana Baramidze, Senior Economic and Environmental Officer at the OSCE Centre. “The OSCE Centre in Bishkek fully supports the efforts of the Kyrgyz Government in its attempt to establish effective systems to counter money laundering and the financing of terrorism.”

Chyngyz Kenenbayev, Head of the Organizational and Legal department of the SFIS, said: “We are holding this educational seminar to train representatives of national authorities and the private sector so that we can successfully pass the second round of the evaluation by the Eurasian Group on Combating Money Laundering and Financing of Terrorism (EAG). In order to fulfil international obligations under the EAG, the Kyrgyz Republic is to conduct a mutual assessment of its anti-money laundering and combating the financing of terrorism system. From 28 November to 9 December 2016, a visiting mission of EAG evaluators will conduct this assessment within the Financial Action Task Force (FATF) initiative."

The training course, which was conducted by two IMF experts and three SFIS officials, was part of the OSCE assistance to the Kyrgyz Government in preparing for the second round of the FATF evaluation.




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Rep. Michael McCaul says he was detained at Dulles airport over being 'disoriented'

Republican Rep. Michael McCaul, the chairman of the House Foreign Affairs Committee, said he was detained by police at an airport near Washington, D.C. earlier this month.




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Modeling Quality Attributes with Aspect-Oriented Architectural Templates

The quality attributes of a software system are, to a large extent, determined by the decisions taken early in the development process. Best practices in software engineering recommend the identification of important quality attributes during the requirements elicitation process, and the specification of software architectures to satisfy these requirements. Over the years the software engineering community has studied the relationship between quality attributes and the use of particular architectural styles and patterns. In this paper we study the relationship between quality attributes and Aspect-Oriented Software Architectures - which apply the principles of Aspect-Oriented Software Development (AOSD) at the architectural level. AOSD focuses on identifying, modeling and composing crosscutting concerns - i.e. concerns that are tangled and/or scattered with other concerns of the application. In this paper we propose to use AO-ADL, an aspect-oriented architectural description language, to specify quality attributes by means of parameterizable, and thus reusable, architectural patterns. We particularly focus on quality attributes that: (1) have major implications on software functionality, requiring the incorporation of explicit functionality at the architectural level; (2) are complex enough as to be modeled by a set of related concerns and the compositions among them, and (3) crosscut domain specific functionality and are related to more than one component in the architecture. We illustrate our approach for usability, a critical quality attribute that satisfies the previous constraints and that requires special attention at the requirements and the architecture design stages.




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An Aspect-Oriented Framework for Weaving Domain-Specific Concerns into Component-Based Systems

Software components are used in various application domains, and many component models and frameworks have been proposed to fulfill domain-specific requirements. The general trend followed by these approaches is to provide ad-hoc models and tools for capturing these requirements and for implementing their support within dedicated runtime platforms, limited to features of the targeted domain. The challenge is then to propose more flexible solutions, where components reuse is domain agnostic. In this article, we present a framework supporting compositional construction and development of applications that must meet various extra-functional/domain-specific requirements. The key points of our contribution are: i) We target development of component-oriented applications where extra-functional requirements are expressed as annotations on the units of composition in the application architecture. ii) These annotations are implemented as open and extensible component-based containers, achieving full separation of functional and extra-functional concerns. iii) Finally, the full machinery is implemented using the Aspect-Oriented Programming paradigm. We validate our approach with two case studies: the first is related to real-time and embedded applications, while the




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Service Oriented Multimedia Delivery System in Pervasive Environments

Service composition is an effective approach for large-scale multimedia delivery. In previous works, user requirement is represented as one fixed functional path which is composed of several functional components in a certain order. Actually, there may be several functional paths (deliver different quality level multimedia data, e.g., image pixel, frame rate) that can meet one request. And due to the diversity of devices and connections in pervasive environment, system should choose a suitable media quality delivery path in accordance with context, instead of one fixed functional path. This paper presents a deep study of multimedia delivery problem and proposes an on-line algorithm LDPath and an off-line centralized algorithm LD/RPath respectively. LDPath aims at delivering multimedia data to end user with lowest delay by choosing services to build delivery paths hop-by-hop, which is adapted to the unstable open environment. And LD/RPath is developed for a relatively stable environment, which generates delivery paths according to the trade-off between delay and reliability metrics, because the service reliability is also an important fact in such scenario. Experimental results show that both algorithms have good performance with low overhead to the system.




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Utilizing BlueJ to Teach Polymorphism in an Advanced Object-Oriented Programming Course




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Activity Oriented Teaching Strategy for Software Engineering Course: An Experience Report

Aim/Purpose: This paper presents the findings of an Activity-Oriented Teaching Strategy (AOTS) conducted for a postgraduate level Software Engineering (SE) course with the aim of imparting meaningful software development experience for the students. The research question is framed as whether the activity-oriented teaching strategy helps students to acquire practical knowledge of Software Engineering and thus bridge the gap between academia and software industry. Background: Software Engineering Education (SEE) in India is mainly focused on teaching theoretical concepts rather than emphasizing on practical knowledge in software development process. It has been noticed that many students of CS/IT background are struggling when they start their career in the software industry due to inadequate familiarity with the software development process. In the current context of SE education, there is a knowledge gap between the theory learned in the classroom and the actual requirement demanded by the software industry. Methodology: The methodology opted for in this study was action research since the teachers are trying to solve the practical problems and deficiencies encountered while teaching SE. There are four pedagogies in AOTS for fulfilling the requirements of the desired teaching strategy. They are flipped classroom, project role-play for developing project artifacts, teaching by example, and student seminars. The study was conducted among a set of Postgraduate students of the Software Engineering programme at Cochin University of Science and Technology, India. Contribution: AOTS can fulfil both academic and industrial requirements by actively engaging the students in the learning process and thus helping them develop their professional skills. Findings: AOTS can be molded as a promising teaching strategy for learning Software Engineering. It focuses on the essential skill sets demanded by the software industry such as communication, problem-solving, teamwork, and understanding of the software development processes. Impact on Society: Activity-oriented teaching strategies can fulfil both academic and industrial requirements by actively engaging the students in the SE learning process and thus helping them in developing their professional skills. Future Research: AOTS can be refined by adding/modifying pedagogies and including different features like an online evaluation system, virtual classroom etc.




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Investigation of user perception of software features for software architecture recovery in object-oriented software

A well-documented architecture can greatly improve comprehension and maintainability. However, shorter release cycles and quick delivery patterns results in negligence of architecture. In such situations, the architecture can be recovered from its current implementation based on considering dependency relations. In literature, structural and semantic dependencies are commonly used software features, and directory information along with co-change/change history information are among rarely utilised software features. But, they are found to help improve architecture recovery. Therefore, we consider investigating various features that may further improve the accuracy of existing architecture recovery techniques and evaluate their feasibility by considering them in different pairs. We compared five state-of-the-art methods under different feature subsets. We identified that two of them commonly outperform others but surprisingly with low accuracy in some evaluations. Further, we propose a new subset of features that reflects more accurate user perceptions and hence, results in improving the accuracy of architecture recovery techniques.




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Location-Oriented Knowledge Management in a Tourism Context: Connecting Virtual Communities to Physical Locations




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A Web Services-Oriented Approach to Unlock Information




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Understanding Information Technology:   What do Graduates from Business-oriented IS Curricula Need to Know?




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Applying and Evaluating Understanding-Oriented ICT User Training in Upper Secondary Education




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Object-Oriented Hypermedia Design and J2EE Technology for Web-based Applications




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So Different Though So Similar? – Or Vice Versa? Exploration of the Logic Programming and the Object-Oriented Programming Paradigms




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The Reference List Formatter: An Object-Oriented Development Project




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A Conceptual Model for the Creation of a Process-Oriented Knowledge Map (POK-Map) and Implementation in an Electric Power Distribution Company

Helping a company organize and capture the knowledge used by its employees and business processes is a daunting task. In this work we examine several proposed methodologies and synthesize them into a new methodology that we demonstrate through a case study of an electric power distribution company. This is a practical research study. First, the research approach for creating the knowledge map is process-oriented and the processes are considered as the main elements of the model. This research was done in four stages: literature review, model editing, model validation and case study. The Delphi method was used for the research model validation. Some of the important outputs of this research were mapping knowledge flows, determining the level of knowledge assets, expert-area knowledge map, preparing knowledge meta-model, and updating the knowledge map according to the company’s processes. Besides identifying, auditing and visualizing tacit and explicit knowledge, this knowledge mapping enables us to analyze the knowledge areas’ situation and subsequently help us to improve the processes and overall performance. So, a process map does knowledge mapping in a clear and accurate frame. Once the knowledge is used in processes, it creates value.




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An Object Oriented Approach to Improve the Precision of Learning Object Retrieval in a Self Learning Environment




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Analyzing the Quality of Students Interaction in a Distance Learning Object-Oriented Programming Discipline

Teaching object-oriented programming to students in an in-classroom environment demands well-thought didactic and pedagogical strategies in order to guarantee a good level of apprenticeship. To teach it on a completely distance learning environment (e-learning) imposes possibly other strategies, besides those that the e-learning model of Open University of Portugal dictates. This article analyses the behavior of the students of the 1st cycle in Computer Science while interacting with the object-oriented programming (OOP) discipline available to them on the Moodle platform. Through the evaluation of the level of interaction achieved in a group of relevant selected actions by the students, it is possible to identify their relevancy to the success of the programming learning process. Data was extracted from Moodle, numerically analyzed, and, with the use of some charts, behavior patterns of students were identified. This paper points out potential new approaches to be considered in e-learning in order to enhance programming learning results, besides confirming a high level of drop-out and a low level of interaction, thus finding no clear correlation between students’ success and the number of online actions (especially in forums), which reveals a possible failure of the main pillar on which the e-learning model relies.




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An Action-Oriented Perspective of Information Systems in Organizations




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On Categorizing the IS Research Literature: User Oriented Perspective




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Knowledge-Oriented Leadership, Psychological Safety, Employee Voice, and Innovation

Aim/Purpose: The truism is that leadership fosters or restricts innovation behaviours in organisations, but the extent to which it does depends on the leadership style in practice. This study focuses on one of the contemporary leadership styles, knowledge-oriented leadership [KOL], which has received scant attention in research. In doing so, the contextual factors of psychological safety [PS] and employee voice [EV] were applied to determine how KOL influences are channeled to innovation at the individual level. Methodology: Data were collected from 347 academic staff in public universities in Southern Nigeria and subjected to a partial least square [PLS] analytical procedure for data treatment and hypotheses testing using the SmartPLS 3 software for variance-based structural equation modelling. Contribution: The study formed an integrated research framework that links knowledge-oriented leadership and innovation by accounting for the contextual mechanisms of psychological safety and employee voice. Findings: The PLS results demonstrated that the knowledge-oriented leadership and innovation relationship was positive and significant, and this relationship was partially mediated by two variables, namely, PS and EV. Furthermore, the two mediating variables channeled KOL’s influence on innovation in a sequence. Recommendation for Researchers: Organisations need to consider the practical application of KOL to improve innovation outcomes considerably. By this, leadership training programs should include modules, courses, or topics on KOL to engender the formation of requisite managerial skills. More so, they should consider the criterion of demonstrable KOL abilities for leadership selection and recruitment. As a personal development initiative, managers can attend leadership development programmes as well as obtain certification in knowledge management to improve their KOL abilities. This initiative should be encouraged and supported by organisations. In all, the human resource management framework should be responsive to the dynamics of the knowledge economy regarding leadership. Given that PS and EV function as mediators, organisations should actively cultivate an environment enabling interpersonal risky behaviours founded on trust, respect, and cooperation and encourage/support employees who demonstrate such behaviour accordingly. In this line, they should create and sustain a supportive environment that positively reinforces voice decisions and behaviours. Future Research: The study only determined the links between KOL, PS, EV, and innovation in public universities in Southern Nigeria. Other studies may examine the linkages in other knowledge-intensive organisations as well as expand the geographic scope to make for better generality of findings. Future studies should look at other underlying mechanisms that can affect the KOL-innovation relationship, such as psychological capital, work engagement, work commitment, etc. The role of moderators can be identified and introduced to this integrative framework to demonstrate the conditions affecting the linkages.




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How Aphia—The Platform behind Several Online and Taxonomically Oriented Databases—Can Serve Both the Taxonomic Community and the Field of Biodiversity Informatics




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Public-Private Partnership results in Tourist Oriented Policing Squad

ORLANDO, FL (April 19, 2010)-One of the worlds top tourist and meeting destinations, the International Drive Resort Area, continues with enhanced support of its highly successful prototype safety program, thanks to a grant that has been awarded to Orange County through the U.S. Department of Justice stimulus program.




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Episode 27: Service Oriented Architecture Pt.1

SOA (Service Oriented Architecture) appears to be just another hype - after all we have been building distributed systems for quite a while now. But the real value of SOA is non-technical. In this episode Eberhard and Markus discuss the advantages and disadvantages, what SOA actually is and how it compares to other approaches that have been tried out before.




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Episode 32: Service Oriented Architecture, Pt.2a

In this, as well as in the next episode Eberhard and Markus continue their discussion about SOA (the episode got too long, so we had to split it into two ... SOA 2a and SOA 2b). In this episode, we talk about the various perspectives on SOA (CBD, EAI, BPM), about fundamental requirements towards an SOA, and we discuss the role of models in defining sustainable architectures. We also discuss how a programming model based on the described approach typically looks like. We then discuss a number of issues any large-scale SOA faces (and for which the SOA paradigm does not really provide an out-of-the-box solution: In this episode we discuss data type ownership and (weak) typing of data types.




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Episode 33: Service Oriented Architecture, Pt.2b

This is the second snippet of the SOA 2 double-episode. Eberhard and Markus continue the discussion with the issue of service reuse and a couple of development process issues. We also look at the duality between infrastructure development and application development in the context of an SOA. We then discuss the great spaghetti misunderstanding :-). We conclude this episode with a look at how to integrate BPM into the conceptual SOA framework we've built up to now, and we'll also briefly skim over a number of technologies related to SOA. Note that this episode, as well as the last one, is based on a set of slides; these can be downloaded from here. This episode covers slides 39 through 74.




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Episode 172: Feature-Oriented Software Development with Sven Apel – Pt 1

Sven Apel explains why developing software in a feature-oriented manner is so vital for us as software engineers and why objects are simply not enough.




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Episode 173: Feature-Oriented Software Development with Sven Apel – Pt 2

Recording Venue: University of Passau Guest: Sven Apel Host: Stefan In this second episode on Feature-Oriented Software Development (FOSD), Sven Apel gives us an overview of programming language and tool support for FOSD. He introduces the Eclipse-based FeatureIDE which covers important phases of the FOSD process, namely domain implementation as well as configuration and generation. […]




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How Oriented HDPE Films Enable Mono-material Pouches and Wrappers

The latest advances in high-density polyethylene (HDPE) resin and production technology are making it possible to design all-PE structures for even the most demanding applications.




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OSCE supports workshop on risks-oriented approach in combating money laundering and financing of terrorism in Kyrgyzstan

ISSYK-KUL, Kyrgyzstan, 8 July 2016 – A five-day training course supported by the OSCE Centre in Bishkek on a risk-oriented approach in supervising activities related to anti-money laundering and combating the financing of terrorism concluded today in Bosteri village, in the Issyk-Kul region.

The aim of the workshop, which was organized by the State Financial Intelligence Service under the Government of the Kyrgyz Republic (SFIS) in partnership with the International Monetary Fund (IMF), was to update the relevant State agencies and financial institutions on the practical aspects of a risks-oriented approach in their work. Some 46 officials from law enforcement agencies, supervisory agencies and financial institutions took part.

“A risks-oriented approach is the process which allows the countries to identify, assess and understand risks to effectively combat money laundering and financing of terrorism,” said Nana Baramidze, Senior Economic and Environmental Officer at the OSCE Centre. “The OSCE Centre in Bishkek fully supports the efforts of the Kyrgyz Government in its attempt to establish effective systems to counter money laundering and the financing of terrorism.”

Chyngyz Kenenbayev, Head of the Organizational and Legal department of the SFIS, said: “We are holding this educational seminar to train representatives of national authorities and the private sector so that we can successfully pass the second round of the evaluation by the Eurasian Group on Combating Money Laundering and Financing of Terrorism (EAG). In order to fulfil international obligations under the EAG, the Kyrgyz Republic is to conduct a mutual assessment of its anti-money laundering and combating the financing of terrorism system. From 28 November to 9 December 2016, a visiting mission of EAG evaluators will conduct this assessment within the Financial Action Task Force (FATF) initiative."

The training course, which was conducted by two IMF experts and three SFIS officials, was part of the OSCE assistance to the Kyrgyz Government in preparing for the second round of the FATF evaluation.

Related Stories




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OSCE supports workshop on risks-oriented approach in combating money laundering and financing of terrorism in Kyrgyzstan

ISSYK-KUL, Kyrgyzstan, 8 July 2016 – A five-day training course supported by the OSCE Centre in Bishkek on a risk-oriented approach in supervising activities related to anti-money laundering and combating the financing of terrorism concluded today in Bosteri village, in the Issyk-Kul region.

The aim of the workshop, which was organized by the State Financial Intelligence Service under the Government of the Kyrgyz Republic (SFIS) in partnership with the International Monetary Fund (IMF), was to update the relevant State agencies and financial institutions on the practical aspects of a risks-oriented approach in their work. Some 46 officials from law enforcement agencies, supervisory agencies and financial institutions took part.

“A risks-oriented approach is the process which allows the countries to identify, assess and understand risks to effectively combat money laundering and financing of terrorism,” said Nana Baramidze, Senior Economic and Environmental Officer at the OSCE Centre. “The OSCE Centre in Bishkek fully supports the efforts of the Kyrgyz Government in its attempt to establish effective systems to counter money laundering and the financing of terrorism.”

Chyngyz Kenenbayev, Head of the Organizational and Legal department of the SFIS, said: “We are holding this educational seminar to train representatives of national authorities and the private sector so that we can successfully pass the second round of the evaluation by the Eurasian Group on Combating Money Laundering and Financing of Terrorism (EAG). In order to fulfil international obligations under the EAG, the Kyrgyz Republic is to conduct a mutual assessment of its anti-money laundering and combating the financing of terrorism system. From 28 November to 9 December 2016, a visiting mission of EAG evaluators will conduct this assessment within the Financial Action Task Force (FATF) initiative."

The training course, which was conducted by two IMF experts and three SFIS officials, was part of the OSCE assistance to the Kyrgyz Government in preparing for the second round of the FATF evaluation.

Related Stories




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[ E.800SerSup10 (01/16) ] - ITU-T E.800 series - QoS/QoE framework for the transition from network oriented to service oriented operations

ITU-T E.800 series - QoS/QoE framework for the transition from network oriented to service oriented operations




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[ P.811 (01/19) ] - Subjective test methodology for evaluating Speech oriented stereo communication systems over headphones

Subjective test methodology for evaluating Speech oriented stereo communication systems over headphones




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U4SSC - User guide for the toolkit on digital transformation for people-oriented cities and communities

U4SSC - User guide for the toolkit on digital transformation for people-oriented cities and communities




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U4SSC - User guide for the toolkit on digital transformation for people-oriented cities and communities - Version 2

U4SSC - User guide for the toolkit on digital transformation for people-oriented cities and communities - Version 2




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[ Z.161.7 (10/19) ] - Testing and Test Control Notation version 3: TTCN-3 language extensions: Object-oriented features

Testing and Test Control Notation version 3: TTCN-3 language extensions: Object-oriented features




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[ Z.107 (10/19) ] - Specification and Description Language - Object-oriented data in SDL-2010

Specification and Description Language - Object-oriented data in SDL-2010




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[ Z.107 (06/21) ] - Specification and Description Language - Object-oriented data in SDL-2010

Specification and Description Language - Object-oriented data in SDL-2010




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[ Z.161.7 (10/21) ] - Testing and Test Control Notation version 3: TTCN-3 language extensions: Object-oriented features

Testing and Test Control Notation version 3: TTCN-3 language extensions: Object-oriented features




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[ Z.161.7 (10/22) ] - Testing and Test Control Notation version 3: TTCN-3 Language Extensions: Object-oriented features

Testing and Test Control Notation version 3: TTCN-3 Language Extensions: Object-oriented features




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Reskilling the workforce using technology-oriented training: A conversation with Stacie Petter and Laurie Giddens

How can technology-oriented training programs help address information technology skills gaps amongst the government workforce? How can agencies achieve effective technology-oriented training outcomes when reskilling employees? What lessons can be learned from the successful use of technology-oriented training programs? Join host Michael Keegan as he explores these questions and more with Professors Stacie Petter and Laurie Giddens authors of the IBM Center report, Reskilling the Workforce with Technology-Oriented Training on the next The Business of Government Hour.

The post Reskilling the workforce using technology-oriented training: A conversation with Stacie Petter and Laurie Giddens first appeared on Federal News Network.




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Service-Oriented Computing – ICSOC 2015 Workshops WESOA, RMSOC, ISC, DISCO, WESE, BSCI, FOR-MOVES, Goa, India, November 16-19, 2015, Revised Selected Papers

Location: Electronic Resource- 




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Crisis en Oriente Próximo, ¿es posible evitar una guerra a gran escala en la región?

Panelistas consideran que el panorama a futuro depende del rol de China y Rusia. También analizaron el panorama del acuerdo nacional y los debates que se desarrollan en el Congreso.




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Bogotá sabe a Llanos: el festival que enaltece la gastronomía del oriente




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FAFO: Latino Believes Trump Won't Deport Family-Oriented Migrants

In today's edition of FAFO (F*ck Around and Find Out) we have a man who is probably going to learn the hard way that being one of the "good ones" means nothing to the Trump administration.

See, MAGA loyalty only goes one way. They want your vote, but won't do anything to make your life better. This Latino male Trump voter told CNN that mass deportation won't extend to law-abiding workers.

He actually said, with a straight face: “That wouldn’t be fair. They need to make sure that they don’t throw away, they don’t kick out, they don’t deport people that are family oriented.”

Sweetie, they don't care if you are family oriented or not. If you are working a good job or not. If you are a criminal or not. To them, being non-American is enough to get you kicked out (and probably put in a camp before). Enjoy that Trump hair visor hat, honey. The leopards are coming for your face.

"Fucketh around and ye shall findeth out" - A Twitter User in 2024

read more





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Lipid and Metabolic Syndrome Traits in Coronary Artery Disease: A Mendelian Randomization Study [Patient-Oriented and Epidemiological Research]

Mendelian randomization (MR) of lipid traits in coronary artery disease (CAD) has provided evidence for causal associations of low-density lipoprotein cholesterol (LDL-C) and triglycerides (TG) in CAD, but many lipid trait genetic variants have pleiotropic effects on other cardiovascular risk factors that may bias MR associations. The goal of this study was to evaluate pleiotropic effects of lipid trait genetic variants and to account for these effects in MR of lipid traits in CAD. We performed multivariable MR using inverse variance-weighted (IVW) and MR-Egger methods in large (n ≥ 300,000) GWAS datasets. We found that 30% of lipid trait genetic variants have effects on metabolic syndrome traits, including body mass index (BMI), type 2 diabetes (T2D), and systolic blood pressure (SBP). Nonetheless, in multivariable MR analysis, LDL-C, high-density lipoprotein cholesterol (HDL-C), TG, BMI, T2D, and SBP are independently associated with CAD, and each of these associations is robust to adjustment for directional pleiotropy. MR at loci linked to direct effects on HDL-C and TG suggests locus- and mechanism-specific causal effects of these factors on CAD.




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Esterification of 4{beta}-hydroxycholesterol and other oxysterols in human plasma occurs independently of LCAT [Patient-Oriented and Epidemiological Research]

The acyltransferase LCAT mediates FA esterification of plasma cholesterol. In vitro studies have shown that LCAT also FA-esterifies several oxysterols, but in vivo evidence is lacking. Here, we measured both free and FA-esterified forms of sterols in 206 healthy volunteers and 8 individuals with genetic LCAT deficiency, including familial LCAT deficiency (FLD) and fish-eye disease (FED). In the healthy volunteers, the mean values of the ester-to-total molar ratios of the following sterols varied: 4β-hydroxycholesterol (4βHC), 0.38; 5,6α-epoxycholesterol (5,6αEC), 0.46; 5,6β-epoxycholesterol (5,6βEC), 0.51; cholesterol, 0.70; cholestane-3β,5α,6β-triol (CT), 0.70; 7-ketocholesterol (7KC), 0.75; 24S-hydroxycholesterol (24SHC), 0.80; 25-hydroxycholesterol (25HC), 0.81; 27-hydroxycholesterol (27HC), 0.86; and 7α-hydroxycholesterol (7αHC), 0.89. In the individuals with LCAT deficiency, the plasma levels of the FA-esterified forms of cholesterol, 5,6αEC, 5,6βEC, CT, 7αHC, 7KC, 24SHC, 25HC, and 27HC, were significantly lower than those in the healthy volunteers. The individuals with FLD had significantly lower FA-esterified forms of 7αHC, 24SHC, and 27HC than those with FED. It is of note that, even in the three FLD individuals with negligible plasma cholesteryl ester, substantial amounts of the FA-esterified forms of 4βHC, 5,6αEC, 7αHC, 7KC, and 27HC were present. We conclude that LCAT has a major role in the FA esterification of many plasma oxysterols but contributes little to the FA esterification of 4βHC. Substantial FA esterification of 4βHC, 5,6αEC, 7αHC, 7KC, and 27HC is independent of LCAT.




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Genetic susceptibility, dietary cholesterol intake, and plasma cholesterol levels in a Chinese population [Patient-Oriented and Epidemiological Research]

Accompanied with nutrition transition, non-HDL-C levels of individuals in Asian countries has increased rapidly, which has caused the global epicenter of nonoptimal cholesterol to shift from Western countries to Asian countries. Thus, it is critical to underline major genetic and dietary determinants. In the current study of 2,330 Chinese individuals, genetic risk scores (GRSs) were calculated for total cholesterol (TC; GRSTC, 57 SNPs), LDL-C (GRSLDL-C, 45 SNPs), and HDL-C (GRSHDL-C, 65 SNPs) based on SNPs from the Global Lipid Genetics Consortium study. Cholesterol intake was estimated by a 74-item food-frequency questionnaire. Associations of dietary cholesterol intake with plasma TC and LDL-C strengthened across quartiles of the GRSTC (effect sizes: –0.29, 0.34, 2.45, and 6.47; Pinteraction = 0.002) and GRSLDL-C (effect sizes: –1.35, 0.17, 5.45, and 6.07; Pinteraction = 0.001), respectively. Similar interactions with non-HDL-C were observed between dietary cholesterol and GRSTC (Pinteraction = 0.001) and GRSLDL-C (Pinteraction = 0.004). The adverse effects of GRSTC on TC (effect sizes across dietary cholesterol quartiles: 0.51, 0.82, 1.21, and 1.31; Pinteraction = 0.023) and GRSLDL-C on LDL-C (effect sizes across dietary cholesterol quartiles: 0.66, 0.52, 1.12, and 1.56; Pinteraction = 0.020) were more profound in those having higher cholesterol intake compared with those with lower intake. Our findings suggest significant interactions between genetic susceptibility and dietary cholesterol intake on plasma cholesterol profiles in a Chinese population.