α-Linked galactose is a common carbohydrate motif in nature that is processed by a variety of glycoside hydrolases from different families. Terminal Galα1–3Gal motifs are found as a defining feature of different blood group and tissue antigens, as well as the building block of the marine algal galactan λ-carrageenan. The blood group B antigen and linear α-Gal epitope can be processed by glycoside hydrolases in family GH110, whereas the presence of genes encoding GH110 enzymes in polysaccharide utilization loci from marine bacteria suggests a role in processing λ-carrageenan. However, the structure–function relationships underpinning the α-1,3-galactosidase activity within family GH110 remain unknown. Here we focus on a GH110 enzyme (PdGH110B) from the carrageenolytic marine bacterium Pseudoalteromonas distincta U2A. We showed that the enzyme was active on Galα1–3Gal but not the blood group B antigen. X-ray crystal structures in complex with galactose and unhydrolyzed Galα1–3Gal revealed the parallel β-helix fold of the enzyme and the structural basis of its inverting catalytic mechanism. Moreover, an examination of the active site reveals likely adaptations that allow accommodation of fucose in blood group B active GH110 enzymes or, in the case of PdGH110, accommodation of the sulfate groups found on λ-carrageenan. Overall, this work provides insight into the first member of a predominantly marine clade of GH110 enzymes while also illuminating the structural basis of α-1,3-galactoside processing by the family as a whole.

Systemic antibody light chains (AL) amyloidosis is characterized by deposition of amyloid fibrils derived from a particular antibody light chain. Cardiac involvement is a major risk factor for mortality. Using MAS solid-state NMR, we studied the fibril structure of a recombinant light chain fragment corresponding to the fibril protein from patient FOR005, together with fibrils formed by protein sequence variants that are derived from the closest germline (GL) sequence. Both analyzed fibril structures were seeded with ex-vivo amyloid fibrils purified from the explanted heart of this patient. We find that residues 11-42 and 69-102 adopt β-sheet conformation in patient protein fibrils. We identify arginine-49 as a key residue that forms a salt bridge to aspartate-25 in the patient protein fibril structure. In the germline sequence, this residue is replaced by a glycine. Fibrils from the GL protein and from the patient protein harboring the single point mutation R49G can be both heterologously seeded using patient ex-vivo fibrils. Seeded R49G fibrils show an increased heterogeneity in the C-terminal residues 80-102, which is reflected by the disappearance of all resonances of these residues. By contrast, residues 11-42 and 69-77, which are visible in the MAS solid-state NMR spectra, show 13Cα chemical shifts that are highly like patient fibrils. The mutation R49G thus induces a conformational heterogeneity at the C terminus in the fibril state, whereas the overall fibril topology is retained. These findings imply that patient mutations in FOR005 can stabilize the fibril structure.

Penn State Schuylkill’s Alpha Epsilon Lambda chapter of Lambda Pi Eta, the national communication honor society, inducted eight new members in a ceremony held at the Michael C. Fryer Conference Center on Oct. 16. Lambda Pi Eta, founded in 1985, recognizes academic excellence in communication students and promotes professional development.

We use the classical Fourier analysis to introduce analytic families of weighted differential operators on the unit sphere. These operators are polynomial functions of the usual Beltrami-Laplace operator. New inversion formulas are obtained for totally geodesic Funk transforms on the sphere and the correpsonding lambda-cosine transforms.

Lambda functions are quite an intuitive concept of Modern C++ introduced in C++11, so there are already tons of articles on lambda function tutorials over the internet. But still, there are some untold things (like IIFE, types of lambda, etc.) left, which nobody talks about. Therefore, here I am to not only show you lambda function in C++, but we'll also cover how it works internally and other aspects of Lambda.

The title of this article is a bit misleading. Because lambda doesn't always synthesize to function pointer. It's an expression (precisely unique closure). But I have kept it that way for simplicity. So from now on, I will use lambda function and expression interchangeably.

Graduating members of Penn State Scranton’s Lambda Pi Eta Alpha Epsilon Pi chapter received their honor cords recently during a virtual celebration. Lambda Pi Eta is the official honor society of the National Communication Association and currently, the only academic honor society at Penn State Scranton.

And it’s not enough until the circle of opportunity and equality is so wide – and so accessible – that the American Dream is available to everyone with the heart to dream it.

Online Resource