[This Day] Fireworks will be at crescendo when Varsity Cup Men's Football 2024 season comes to an end this Wednesday, November 13, 2024 at The Main Bowl of the University of Benin Sport Complex, Benin City, Edo State.

Fashion Dot showcased its advanced tailoring CAD software and laser cutting machines at GTE 2024. These machines allow tailors to input measurements and auto-generate cutting diagrams, improving accuracy, reducing costs, and eliminating manual work. The company offers customer support and regularly updates its software to include new garment models.

Two young Bangladeshi women experienced great joy and release from the OM tailoring courses, bringing dignity, honour and financial gain to their communities and families.

Despite the warning by the Kwara Police Command to market leaders in the state to comply strictly with the guidelines on the relaxed COVID-19 lockdown order, traders and buyers have refused to observe the safety protocols to curb the spread of the virus. DAILY POST visit to Ipata market among others in Ilorin, the state […]

COVID-19: Traders, buyers shun face masks, social distancing orders in Ilorin

New research has shown that policy measures in Denmark have successfully reduced total nitrogen loading to 10 estuaries by 39% in recent decades. However, to fully meet the targets of the EU Nitrates Directive and comply with the EU Water Framework Directive in a cost-effective manner, further mitigation measures must be tailored and focused to particular areas.

The cost-effectiveness of agri-environment schemes to conserve species and habitats under the Scottish Rural Development Programme (SRDP) has been assessed by a recent study. Cost-effectiveness was found to vary widely between schemes. Improvements in cost-effectiveness relative to specific conservation objectives might be achieved with increased geographical targeting, advice and monitoring of impacts.

Two young Bangladeshi women experienced great joy and release from the OM tailoring courses, bringing dignity, honour and financial gain to their communities and families.

Title: Tailoring Exercise to Your Age
Category: Health News
Created: 4/29/2019 12:00:00 AM
Last Editorial Review: 4/29/2019 12:00:00 AM

ABSTRACT

Pathogenicity islands and plasmids bear genes for pathogenesis of various Escherichia coli pathotypes. Although there is a basic understanding of the contribution of these virulence factors to disease, less is known about variation in regulatory networks in determining disease phenotypes. Here, we dissected a regulatory network directed by the conserved iron homeostasis regulator, ferric uptake regulator (Fur), in uropathogenic E. coli (UPEC) strain CFT073. Comparing anaerobic genome-scale Fur DNA binding with Fur-dependent transcript expression and protein levels of the uropathogen to that of commensal E. coli K-12 strain MG1655 showed that the Fur regulon of the core genome is conserved but also includes genes within the pathogenicity/genetic islands. Unexpectedly, regulons indicative of amino acid limitation and the general stress response were also indirectly activated in the uropathogen fur mutant, suggesting that induction of the Fur regulon increases amino acid demand. Using RpoS levels as a proxy, addition of amino acids mitigated the stress. In addition, iron chelation increased RpoS to the same levels as in the fur mutant. The increased amino acid demand of the fur mutant or iron chelated cells was exacerbated by aerobic conditions, which could be partly explained by the O₂-dependent synthesis of the siderophore aerobactin, encoded by an operon within a pathogenicity island. Taken together, these data suggest that in the iron-poor environment of the urinary tract, amino acid availability could play a role in the proliferation of this uropathogen, particularly if there is sufficient O₂ to produce aerobactin.

IMPORTANCE Host iron restriction is a common mechanism for limiting the growth of pathogens. We compared the regulatory network controlled by Fur in uropathogenic E. coli (UPEC) to that of nonpathogenic E. coli K-12 to uncover strategies that pathogenic bacteria use to overcome iron limitation. Although iron homeostasis functions were regulated by Fur in the uropathogen as expected, a surprising finding was the activation of the stringent and general stress responses in the uropathogen fur mutant, which was rescued by amino acid addition. This coordinated global response could be important in controlling growth and survival under nutrient-limiting conditions and during transitions from the nutrient-rich environment of the lower gastrointestinal (GI) tract to the more restrictive environment of the urinary tract. The coupling of the response of iron limitation to increased demand for amino acids could be a critical attribute that sets UPEC apart from other E. coli pathotypes.