Sobre Besteiro

Lo peor no es que el ayudante del mago más famosos del mundo sea un farsante, sino el silencio cómplice y decepcionante de los que no le piden una explicación.

Mariana Castañeda compartió algunos recuerdos en 6AM de su padre, el reconocido productor de Caracol Radio que trabajó de la mano de históricos como Yamid Amat y Darío Arizmendi

El gobernador del Vichada, Álex Benito habló en 6AM sobre la caída del helicóptero de la Fuerza Aérea, que enluta nuevamente al país.

Ante los acontecimientos que afectan a esta ciudad, se confirmó que Nelson Quijano fue el primer colombiano fallecido por las inundaciones. Su hermano habló en 6AM.

End-to-end RNA-sequencing methods that capture 5'-sequence content without cumbersome library manipulations are of great interest, particularly for analysis of long RNAs. While template-switching methods have been developed for RNA sequencing by distributive short-read RTs, such as the MMLV RTs used in SMART-Seq methods, they have not been adapted to leverage the power of ultraprocessive RTs, such as those derived from group II introns. To facilitate this transition, we dissected the individual processes that guide the enzymatic specificity and efficiency of the multistep template-switching reaction carried out by RTs, in this case, by MarathonRT. Remarkably, this is the first study of its kind, for any RT. First, we characterized the nucleotide specificity of nontemplated addition (NTA) reaction that occurs when the RT extends past the RNA 5'-terminus. We then evaluated the binding specificity of specialized template-switching oligonucleotides, optimizing their sequences and chemical properties to guide efficient template-switching reaction. Having dissected and optimized these individual steps, we then unified them into a procedure for performing RNA sequencing with MarathonRT enzymes, using a well-characterized RNA reference set. The resulting reads span a six-log range in transcript concentration and accurately represent the input RNA identities in both length and composition. We also performed RNA-seq from total human RNA and poly(A)-enriched RNA, with short- and long-read sequencing demonstrating that MarathonRT enhances the discovery of unseen RNA molecules by conventional RT. Altogether, we have generated a new pipeline for rapid, accurate sequencing of complex RNA libraries containing mixtures of long RNA transcripts.