Ion mobility separates molecules in the gas-phase based on their physico-chemical properties, providing information about their size as collisional cross-sections. The timsTOF Pro combines trapped ion mobility with a quadrupole, collision cell and a TOF mass analyzer, to probe ions at high speeds with on-the-fly fragmentation. Here, we show that on this platform ion mobility is beneficial for cross-linking MS (XL-MS). Cross-linking reagents covalently link amino acids in proximity, resulting in peptide pairs after proteolytic digestion. These cross-linked peptides are typically present at low abundance in the background of normal peptides, which can partially be resolved by using enrichable cross-linking reagents. Even with a very efficient enrichable cross-linking reagent, like PhoX, the analysis of cross-linked peptides is still hampered by the co-enrichment of peptides connected to a partially hydrolyzed reagent – termed mono-linked peptides. For experiments aiming to uncover protein-protein interactions these are unwanted byproducts. Here, we demonstrate that gas-phase separation by ion mobility enables the separation of mono-linked peptides from cross-linked peptide pairs. A clear partition between these two classes is observed at a CCS of 500 Ų and a monoisotopic mass of 2 kDa, which can be used for targeted precursor selection. A total of 50-70% of the mono-linked peptides are prevented from sequencing, allowing the analysis to focus on sequencing the relevant cross-linked peptide pairs. In applications to both simple proteins and protein mixtures and a complete highly complex lysate this approach provides a substantial increase in detected cross-linked peptides.

National Bureau of Economic Research

A device including one or more layers with lateral regions configured to facilitate the transmission of radiation through the layer and lateral regions configured to facilitate current flow through the layer is provided. The layer can comprise a short period superlattice, which includes barriers alternating with wells. In this case, the barriers can include both transparent regions, which are configured to reduce an amount of radiation that is absorbed in the layer, and higher conductive regions, which are configured to keep the voltage drop across the layer within a desired range.

Wind turbine rotor blades include a shell having a leading edge opposite a trailing edge, a structural support member that supports the shell and is disposed internal the wind turbine rotor blade between the leading edge and the trailing edge and extends for at least a portion of a rotor blade span length, and a resistive cellular support structure disposed at least partially about the wind turbine rotor blade that physically supports at least a portion of the wind turbine rotor blade and at least partially absorbs radar energy.

Wind turbine rotor blades with a reduced radar cross sections include a shell having a leading edge opposite a trailing edge, a structural support member that supports the shell and is disposed internal the wind turbine rotor blade between the leading edge and the trailing edge and extends for at least a portion of a rotor blade span length, wherein the structural support member comprises fiberglass, one or more cavities internal the wind turbine rotor blade, and a lightweight broadband radar absorbing filler material disposed in at least one of the one or more cavities to provide the reduced radar cross section.