cornavirus

Assessment of optimized FRET substrates as universal corona- and picornavirus main protease substrates for screening assays

RSC Adv., 2024, 14,35438-35446
DOI: 10.1039/D4RA06573E, Paper
Open Access
  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Conrad Fischer, Tayla J. Van Oers, Marco J. van Belkum, Tess Lamer, Aaron Romney, Pu Chen, M. Joanne Lemieux, John C. Vederas
A short FRET peptide was identified as a universal substrate for selected coronavirus Mpros. Kinetic parameters, buffer stability and Z'-assay suggest this substrate as an excellent target for CoV-Mpro HT inhibitor screening assay development.
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cornavirus

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