We all have those moments, when you walk into a room and forget why you're there, or when you're trying to focus, but your mind keeps wandering. It happens to the best of us. With so many distractions and things on

Manchester : Manchester University Press, 2020.

Analysts said Mr. Yoon may seek to find a way to capitalise on a personal friendship with Mr. Trump to advance Seoul's interests as Trump's "America First" foreign policy plan

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Berlin : A. Hirschwald, 1870.

Vampire bats are social creatures that build relationships through grooming and food-sharing, but when they feel ill, they self-isolate and call out for contact far less

Vampire bats are social creatures that build relationships through grooming and food-sharing, but when they feel ill, they self-isolate and call out for contact far less

The Duchess of Cornwall has revealed she has taken up ballet and is practising her poses and positions to keep fit in lockdown.

"One concrete and very direct result of the Justice Department’s participation in the Task Force is that we have forged and fostered strong relationships with our partners in the fight against financial fraud," said Assistant Attorney General Breuer.

Vampire bats are social creatures that build relationships through grooming and food-sharing, but when they feel ill, they self-isolate and call out for contact far less

DOMINIC LAWSON: So that's clear: it is absolutely unacceptable for anyone with eugenicist views to have a position of the slightest influence within British politics.

Liverpool defender Alexander-Arnold effortlessly caressed the ball across his garden into his garden lounger while Lingard punted the ball into a black bin from an elevated position.

Helen Flanagan has revealed she's staying sane during lockdown by going running and practising yoga.

EXCLUSIVE: The Government are set to recommend that all UK sportspeople agree a coronavirus charter before returning to competitive action, Sportsmail understands.

As the world enters a state of lockdown in a bid to combat the coronavirus pandemic, many people have been told to work from home in self-isolation to reduce the deadly virus' spread.

The former Saturdays star was given a helping hand by boyfriend Stuart Broad as the couple kept themselves entertained during an extended period of self-quarantine.

The 2008 Champions League final between Chelsea and Manchester United could've gone very differently if everything had gone according to John Terry's training plan. 

RB Leipzig’s Julian Nagelsmann, whose side will be chasing a first Bundesliga title when the German league resumes next weekend, is to practise coaching while wearing a face mask in order to be ready for a return to action.

Dewey Library - JZ1570.A57 R843 2018

Hayden Library - PN1995.9.D6 K57 2018

Serisier, Ian David, author

Infosys executive chairman Narayana Murthy said that the Aam Aadmi Party need to need to use to modern social principles and technology to get back the lost attractiveness. "They should stop practising economics of 1940s and 1950s," he said.

Australia. Parliament. Senate. Community Affairs References Committee, author, issuing body

Dewey Library - K3585.S8673 2020

Rotch Library - DT96.B447 2016